2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS:2816-24-2

Enzyme mkpụrụ: ONPG na-ejikarị dị ka mkpụrụ maka beta-galactosidase, enzyme na hydrolyzes ONPG na-emepụta a-acha odo odo compound (o-nitrophenol) nke nwere ike mfe ịhụ spectrophotometrically.Enwere ike iji mmeghachi omume enzymatic a tụọ ọrụ beta-galactosidase, na-eme ONPG ka ọ bụrụ ngwa bara uru na ọmụmụ ihe ọmụmụ.

Nnwale usoro ihe ọmụmụ ihe omimi: ONPG na-eji dị ka mkpụrụ n'ime nyocha ihe omimi dị iche iche, ọkachasị na nyocha mkpụrụ ndụ beta-galactosidase.N'ime nyocha ndị a, a na-eji mkpụrụ dabere na ONPG iji tụọ ọrụ nke mkpụrụ ndụ onye nta akụkọ, bụ nke a na-ahazikarị site na usoro mmasị onye nkwalite.Ọrụ beta-galactosidase, nke ngbanwe agba egosipụtara na ONPG hydrolysis, na-enye ozi bara uru gbasara ọrụ nkwalite.

Nyocha okwu mkpụrụ ndụ: ONPG na-ejikwa nyocha nke mkpụrụ ndụ ihe nketa.Site na ijikọ usoro mkpali nke mmasị na mkpụrụ ndụ beta-galactosidase, ndị nyocha nwere ike tụọ ọrụ beta-galactosidase site na iji ONPG dị ka mkpụrụ.Ọkwa nke ọrụ beta-galactosidase na-egosipụta ike na ọrụ nke onye na-akwalite ya, na-enye ohere maka nyocha nke usoro okwu mkpụrụ ndụ ihe nketa.

Ngwa nyocha: Enwere ike iji ONPG na ngwa nyocha, ọkachasị n'ịchọpụta microorganisms pathogenic.Nje bacteria dị iche iche, dị ka Escherichia coli na ụfọdụ ụdị Shigella na Salmonella, na-emepụta beta-galactosidase nke nwere ike ịgbaji ONPG.Mmeghachi omume hydrolysis a na-emepụta mgbanwe agba a na-ahụ anya, nke enwere ike iji chọpụta ọnụnọ nke nje bacteria ndị a na nlele ụlọ ọgwụ.