2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS:2816-24-2

2-Nitrophenyl-beta-D-glucopyranoside bụ ihe mejupụtara kemịkalụ nke nwere molekul glucopyranoside nke ejikọtara na otu nitrophenyl. A na-ejikarị ya eme ihe dị ka ihe ndabere na nnwale enzymatic iji chọpụta ma tụọ ọrụ nke enzymes dị ka beta-glucosidase. Enwere ike kewaa otu nitrophenyl site na enzyme, na-ebute mwepụta nke ngwaahịa agba odo nke enwere ike ịtụ spectrophotometric. Ihe mejupụtara a bara uru karịsịa n'ịmụ gbasara kinetics enzyme na nyocha dị elu nke ndị na-egbochi enzyme ma ọ bụ ndị na-eme ka ihe rụọ ọrụ. A na-ejikwa ya na nyocha biochemical maka nyocha nke metabolism carbohydrate na dị ka ihe ndabere glycosidic-linkage.

Nye iwu ma ọ bụ rịọ maka ọnụahịa

Nkọwa Ngwaahịa

Akara Ngwaahịa

Ngwa na Mmetụta

Enzyme substrate: A na-ejikarị ONPG eme ihe dị ka ihe ndabere maka beta-galactosidase, enzyme nke na-eme ka ONPG ghara ịdị ọcha iji mepụta ihe mejupụtara agba odo odo (o-nitrophenol) nke enwere ike ịchọpụta ngwa ngwa site na spectrophotometric. Enwere ike iji mmeghachi omume enzymatic a tụọ ọrụ nke beta-galactosidase, na-eme ka ONPG bụrụ ngwa ọrụ bara uru na ọmụmụ enzymology.

Nnyocha bayoloji nke molekụla: A na-eji ONPG eme ihe dị ka ihe ndabere na ọtụtụ nnwale bayoloji nke molekụla, ọkachasị na nnwale mkpụrụ ndụ ihe nketa nke onye na-akọ akụkọ beta-galactosidase. Na nyocha ndị a, a na-eji ihe ndabere dabere na ONPG atụ ọrụ nke mkpụrụ ndụ ihe nketa onye na-akọ akụkọ, nke usoro mmasị pụrụ iche na-achịkwa. Ọrụ beta-galactosidase, nke mgbanwe agba nke emepụtara mgbe ONPG hydrolysis gasịrị, na-enye ozi bara uru gbasara ọrụ onye na-akwalite.

Nyocha nke mkpụrụ ndụ ihe nketa: A na-ejikwa ONPG eme ihe na nyocha nke mkpụrụ ndụ ihe nketa. Site na ijikọ usoro mmasị nke onye na-akwalite ihe na mkpụrụ ndụ ihe nketa beta-galactosidase, ndị nchọpụta nwere ike tụọ ọrụ beta-galactosidase site na iji ONPG dị ka ihe ndabere. Ọkwa nke ọrụ beta-galactosidase na-egosipụta ike na ọrụ nke onye na-akwalite ihe, na-enye ohere maka inyocha ọkwa ngosipụta mkpụrụ ndụ ihe nketa.

Ngwa nchọpụta ọrịa: Enwere ike iji ONPG mee ihe n'inyocha ọrịa, ọkachasị n'ịchọpụta nje ndị na-akpata ọrịa. Ọtụtụ nje bacteria, dị ka Escherichia coli na ụfọdụ ụdị Shigella na Salmonella, na-emepụta beta-galactosidase nke nwere ike ịgbawa ONPG. Mmeghachi omume hydrolysis a na-emepụta mgbanwe agba a na-ahụ anya, nke enwere ike iji chọpụta ọnụnọ nke nje bacteria ndị a na ihe nlele ahụike.

Ihe Nlereanya Ngwaahịa

Nkwakọ ngwaahịa:

Ozi Mgbakwunye:

Ihe mejupụtara ya	C12H15NO8
Nnwale	99%
Ọdịdị	Ntụ ntụ odo dị mfe
Nọmba CAS	2816-24-2
Nkwakọ ngwaahịa	Obere na nnukwu
Ndụ nchekwa	afọ 2
Nchekwa	Debe n'ebe dị jụụ ma kpọọ nkụ
Asambodo	ISO.