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DDT CAS: 3483-12-3 Onye nrụpụta ọnụahịa

DL-Dithiothreitol, makwaara dị ka DTT, bụ ihe na-ebelata ihe a na-ejikarị na nyocha biochemical na molecular biology.Ọ bụ obere molekul nwere otu thiol (sulfur nwere) na nsọtụ ọ bụla.

A na-ejikarị DTT mebie njikọ disulfide na protein, nke na-enyere aka ịgbasa ma ọ bụ mebie ha.Mbelata a nkekọ disulfide dị mkpa na usoro nyocha ụlọ nyocha dị iche iche dị ka nchacha protein, gel electrophoresis na ọmụmụ ihe nhazi protein.A pụkwara iji DTT chebe ìgwè thiol ma gbochie oxidation n'oge usoro nnwale.

A na-agbakwunye DTT na ngwọta nnwale na obere mkpokọta, yana ọrụ ya dabere na ọnụnọ oxygen.Ọ dị mkpa iji nlezianya jikwaa DTT ka ọ na-enwe mmetụta nke ikuku, okpomọkụ, na mmiri, nke nwere ike ibelata irè ya.


Nkọwa ngwaahịa

Mkpado ngwaahịa

Ngwa na Mmetụta

Mbelata nke Bonds Disulfide: A na-ejikarị DTT mebie agbụ disulfide, nke bụ njikọ covalent etolite n'etiti ihe abụọ cysteine ​​dị na protein.Site n'ibelata nkekọ ndị a, DTT na-enyere ndị na-edozi ahụ aka, na-eme ka ọmụmụ ihe nhazi na ọrụ ha.

Mpịakọta protein: DTT nwere ike inye aka na mpịakọta protein kwesịrị ekwesị site na igbochi njikọ disulfide na-ezighi ezi.Ọ na-ebelata njikọ disulfide ọ bụla na-abụghị nke ala nke nwere ike ịmalite n'oge mpịakọta protein, na-ekwe ka protein ahụ nweta ngbanwe nke ala ya.

Ọrụ Enzyme: DTT nwere ike mee ka ụfọdụ enzymes rụọ ọrụ site na ibelata njikọ ọ bụla nke disulfide inhibitory dị ugbu a.Ọzọkwa, DTT nwere ike igbochi oxidation nke cysteine ​​dị mkpa, nke nwere ike ịdị mkpa maka ọrụ enzyme.

Mmepụta Antibody: A na-agbakwunye DTT iji belata njikọ disulfide n'oge mmepụta ọgwụ mgbochi.Ọ na-enyere aka igbochi nguzobe nke njikọ disulfide na-ezighi ezi, nke nwere ike igbochi njikọ antigen kwesịrị ekwesị.

Protein na-eme ka ahụ sie ike: Enwere ike iji DTT mee ka protein kwụsie ike site na igbochi oxidation ma ọ bụ nchịkọta ha.Ọ na-enyere aka ịnọgide na-enwe mbelata nke protein n'oge nchekwa na nnwale usoro.

Mbelata Agents na Molecular Biology: A na-ejikarị DTT mee ihe na usoro ihe ọmụmụ ihe dị iche iche dị ka usoro DNA, PCR, na ịdị ọcha protein.Ọ nwere ike inye aka ịnọgide na-enwe mbelata nke ihe ndị dị oke mkpa, na-ahụ na nsonaazụ nnwale kacha mma.

Nlereanya ngwaahịa

3483-12-3
3483-12-3-2

Nkwakọ ngwaahịa:

6892-68-8-3

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CAS Mba. 3483-12-3
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