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PNPG CAS: 3150-24-1 Onye nrụpụta ọnụahịa
PNPG, ma ọ bụ p-nitrophenyl β-D-glucopyranoside, bụ obere mkpụrụ molekul a na-ejikarị na nyocha biochemical iji tụọ ọrụ nke glucosidase enzymes.Ọ bụ enweghị agba na-abụghị fluorescent, ma n'elu hydrolysis site glucosidase, ọ na-atụgharị na p-nitrophenol, nke nwere a odo agba na-adị mfe spectrophotometrically chọpụta.
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ONPG CAS: 369-07-3 Onye nrụpụta ọnụahịa
O-nitrophenyl-β-D-galactopyranoside (ONPG) bụ ihe sịntetịt eji na biochemical na molecular biology assays iji tụọ ọrụ nke enzyme β-galactosidase.A na-ejikarị ya eme nyocha iji chọpụta mkpụrụ ndụ ihe nketa na sistemu nje, dị ka Escherichia coli.ONPG bụ ihe na-enweghị ụcha nke β-galactosidase gbawara, na-ebute ntọhapụ nke ogige odo, o-nitrophenol.Enwere ike tụọ agba odo na-emepụta spectrophotometrically, na-enye nha nke ọrụ enzyme na-apụtaghị ìhè. A na-akpọkarị nyocha nke na-eji ONPG dị ka nyocha ONPG na bụ usoro a na-ejikarị eme ihe na nchọpụta ihe ọmụmụ ihe omimi iji chọpụta ọkwa ọkwa nke mkpụrụ ndụ ihe nketa nke lac na-achịkwa. operon na sel nje bacteria.
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Nitrotetrazolium Blue Chloride CAS: 298-83-9
Nitrotetrazolium Blue Chloride (NBT) bụ ihe ngosi redox a na-ejikarị na nyocha nke ndu na biochemical.Ọ bụ ntụ ntụ na-acha odo odo na-acha anụnụ anụnụ mgbe ọ na-ebelata, na-eme ka ọ baa uru maka ịchọta ọnụnọ nke ụfọdụ enzymes na ọrụ metabolic.
NBT na-emeghachi omume na ndị na-ebu elektrọn na enzymes dị ka dehydrogenases, nke na-etinye aka na usoro cellular dị iche iche.Mgbe NBT na-ebelata site na enzymes ndị a, ọ na-etolite na-acha anụnụ anụnụ formazan, na-enye ohere maka nchọpụta anya ma ọ bụ spectrophotometric.
A na-ejikarị reagent a na nyocha dị ka nyocha mbelata NBT, ebe a na-eji ya nyochaa ọrụ iku ume nke sel ndị na-alụso ọrịa ọgụ.Enwere ike iji ya mụọ ọrụ enzyme na ụzọ metabolic na nyocha gbasara nrụgide oxidative, ịdị ndụ cell, na ọdịiche cell.
NBT achọtala ngwa na mpaghara dị iche iche, gụnyere microbiology, immunology, na bayoloji cell.Ọ na-agbanwe agbanwe, kwụsiri ike ma dịkwa mfe iji, na-eme ka ọ bụrụ nhọrọ a ma ama maka ọtụtụ ụkpụrụ nnwale.
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Neocuproine CAS: 484-11-7 Onye nrụpụta ọnụahịa
Neocuproine bụ onye na-ere ahịa chelating nke a na-ejikarị na ngwa dị iche iche, gụnyere kemistri nyocha na ọgwụ.Ọ nwere njikọ siri ike maka ion ọla kọpa ma mepụta ogige siri ike na ha.Ngwongwo a na-eme ka neocuproine bara uru maka ịchọpụta na ịkọwapụta ọla kọpa na ngwọta ma ọ bụ ihe nlele.Na mgbakwunye, a mụọla neocuproine maka ngwa ọgwụgwọ nwere ike, ọkachasị n'ịgwọ ọrịa kansa na ọrịa neurodegenerative.
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IPTG CAS: 367-93-1 Onye nrụpụta ọnụahịa
Isopropyl β-D-1-thiogalactopyranoside (IPTG) bụ ihe analọg synthetic nke lactose nke a na-ejikarị na nyocha ihe omimi ihe omimi na ngwa biotechnology.A na-eji IPTG eme ihe n'ụzọ bụ isi iji kpalite ngosipụta nke mkpụrụ ndụ ihe nketa na sistemu nje, ebe ọ na-eje ozi dị ka ihe na-akpali akpali molekụla iji malite ndegharị nke mkpụrụ ndụ ihe nketa.
Mgbe agbakwunyere na usoro uto, IPTG na-ebuli ya site na nje bacteria ma nwee ike jikọta ya na protein lac repressor, na-egbochi ya igbochi ọrụ nke lac operon.Lac operon bụ ụyọkọ mkpụrụ ndụ ihe nketa na-etinye aka na metabolism lactose, na mgbe ewepụrụ protein nke na-akpata repressor, a na-egosipụta mkpụrụ ndụ ihe nketa.
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HATU CAS: 148893-10-1 Onye nrụpụta ọnụahịa
HATU (1-[bis (dimethylamino) methylene] -1H-1,2,3-triazolo [4,5-b] pyridinium 3-oxid hexafluorophosphate) bụ njikọ njikọ nke a na-ejikarị na njikọ peptide na kemịkalụ organic.
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D-fucose CAS: 3615-37-0 Onye nrụpụta ọnụahịa
D-fucose bụ monosaccharide, ọkachasị shuga carbon isii, nke sitere na otu shuga dị mfe a na-akpọ hexoses.Ọ bụ isomer nke glucose, dị iche na nhazi nke otu hydroxyl.
A na-ahụ D-fucose n'ụdị dị iche iche, gụnyere nje bacteria, fungi, osisi na anụmanụ.Ọ na-arụ ọrụ dị mkpa n'ọtụtụ usoro ndu, dị ka akara cell, adhesion cell, na njikọ glycoprotein.Ọ bụ akụkụ nke glycolipids, glycoproteins, na proteoglycans, bụ ndị na-etinye aka na nkwurịta okwu cell na cell na nnabata.
N'ime ụmụ mmadụ, D-fucose na-etinyekwa aka na biosynthesis nke akụkụ glycan dị mkpa, dị ka Lewis antigens na antigens otu ọbara, nke nwere mmetụta na mmịnye ọbara na ndakọrịta nke ọrịa.
Enwere ike nweta D-fucose site na isi mmalite dị iche iche, gụnyere ahihia mmiri, osisi na gbaa ụka microbial.A na-eji ya na nyocha na ngwa biomedical, yana mmepụta nke ụfọdụ ọgwụ na ogige ọgwụgwọ.
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DDT CAS: 3483-12-3 Onye nrụpụta ọnụahịa
DL-Dithiothreitol, makwaara dị ka DTT, bụ ihe na-ebelata ihe a na-ejikarị na nyocha biochemical na molecular biology.Ọ bụ obere molekul nwere otu thiol (sulfur nwere) na nsọtụ ọ bụla.
A na-ejikarị DTT mebie njikọ disulfide na protein, nke na-enyere aka ịgbasa ma ọ bụ mebie ha.Mbelata a nkekọ disulfide dị mkpa na usoro nyocha ụlọ nyocha dị iche iche dị ka nchacha protein, gel electrophoresis na ọmụmụ ihe nhazi protein.A pụkwara iji DTT chebe ìgwè thiol ma gbochie oxidation n'oge usoro nnwale.
A na-agbakwunye DTT na ngwọta nnwale na obere mkpokọta, yana ọrụ ya dabere na ọnụnọ oxygen.Ọ dị mkpa iji nlezianya jikwaa DTT ka ọ na-enwe mmetụta nke ikuku, okpomọkụ, na mmiri, nke nwere ike ibelata irè ya.
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D (+) - Galactose CAS: 59-23-4 Onye nrụpụta ọnụahịa
D- (+) - Galactose bụ shuga monosaccharide na akụkụ dị mkpa nke ọtụtụ usoro ndu.Ọ bụ shuga a na-ahụkarị nke a na-achọta n'ọtụtụ nri, dị ka mkpụrụ osisi, ngwaahịa mmiri ara ehi, na akwụkwọ nri.
Galactose na-enwekarị metabolized n'ime ahụ site na usoro mmeghachi omume enzymatic.Ọ na-arụ ọrụ dị mkpa na nkwurịta okwu cell, mmepụta ume, na biosynthesis nke ụmụ irighiri ihe dị mkpa dị ka glycolipids, glycoproteins, na lactose.
N'ihe gbasara ngwa ya, D- (+) -A na-ejikarị Galactose eme ihe na microbiology na biotechnology dị ka ikuku carbon na mgbasa ozi omenala maka uto nke microorganisms dị iche iche.A na-ejikwa ya na mmepụta nke ogige bioactive dị iche iche, ọgwụ na ngwaahịa nri.Na mgbakwunye, a na-ejikarị ya eme ihe dị ka onye na-achọpụta ọrịa ahụike, ọkachasị n'ule maka nyocha ọrụ imeju na ịchọpụta ọrịa mkpụrụ ndụ ihe nketa metụtara metabolism nke galactose.
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beta-D-Galactose pentaacetate CAS: 4163-60-4
Beta-D-Galactose pentaacetate bụ ngwakọta kemịkalụ sitere na galactose, shuga monosaccharide.Emebere ya site na acetylating nke ọ bụla hydroxyl otu nke molekul galactose nwere otu acetyl ise.
A na-ejikarị onyinye a dị ka ihe nchebe maka galactose na mmeghachi omume kemịkal dị iche iche na usoro mmepụta ihe.Ụdị pentaacetate na-enyere aka ịkwado galactose ma gbochie mmeghachi omume ma ọ bụ mgbanwe na-achọghị n'oge mmeghachi omume.
Tụkwasị na nke a, enwere ike iji ngwakọta a dị ka ihe mmalite maka njikọ nke ihe ndị ọzọ na-emepụta galactose.Enwere ike ịhọpụta otu acetyl dị iche iche iji nweta usoro galactose dị iche iche nwere otu arụrụ arụ ọrụ.
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5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium nnu CAS:129541-41-9
5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium nnu bụ ngwakọta kemịkalụ a na-ejikarị eme nyocha na nyocha ụlọ nyocha.A na-akpọkarị ya X-Gluc ma na-ejikarị ya eme ihe dị ka mkpụrụ maka nchọpụta beta-glucuronidase enzyme ọrụ.
Mgbe beta-glucuronidase dị, ọ na-ekewa njikọ glucuronide na X-Gluc, na-ebute ntọhapụ nke agba anụnụ anụnụ a na-akpọ 5-bromo-4-chloro-3-indolyl.A na-ejikarị mmeghachi omume a na-ahụ anya ma ọ bụ spectrophotometrically chọpụta ngosipụta nke enzyme beta-glucuronidase na sel ma ọ bụ anụ ahụ.
Ụdị nnu sodium nke X-Gluc na-eme ka ọ dịkwuo mfe na ngwọta mmiri, na-eme ka ojiji ya na nyocha ụlọ nyocha.A na-eji X-Gluc eme ihe na nyocha ihe omimi nke mkpụrụ ndụ iji mụọ okwu mkpụrụ ndụ ihe nketa, ọrụ nkwalite, na nyocha mkpụrụ ndụ ndị nta akụkọ.Enwere ike iji ya chọpụta ọnụnọ nke beta-glucuronidase na-emepụta ihe dị ndụ, dị ka ụfọdụ nje bacteria, na nchọpụta microbiological.
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4-Nitrophenyl-beta-D-xylopyranoside CAS: 2001-96-9
4-Nitrophenyl-beta-D-xylopyranoside bụ mkpụrụ chromogenic eji na nyocha enzymatic iji chọpụta na tụọ ọrụ nke enzymes a na-akpọ beta-xylosidases.
