IPTG CAS: 367-93-1 Onye nrụpụta ọnụahịa
Isopropyl β-D-1-thiogalactopyranoside (IPTG) bụ ihe analọg synthetic nke lactose nke a na-ejikarị na nyocha ihe omimi ihe omimi na ngwa biotechnology.A na-eji IPTG eme ihe n'ụzọ bụ isi iji kpalite ngosipụta nke mkpụrụ ndụ ihe nketa na sistemu nje, ebe ọ na-eje ozi dị ka ihe na-akpali akpali molekụla iji malite ndegharị nke mkpụrụ ndụ ihe nketa.
Mgbe agbakwunyere na usoro uto, IPTG na-ebuli ya site na nje bacteria ma nwee ike jikọta ya na protein lac repressor, na-egbochi ya igbochi ọrụ nke lac operon.Lac operon bụ ụyọkọ mkpụrụ ndụ ihe nketa na-etinye aka na metabolism lactose, na mgbe ewepụrụ protein nke na-akpata repressor, a na-egosipụta mkpụrụ ndụ ihe nketa.
A na-ejikarị IPTG na-ejikọta ya na onye na-akwalite mutant lacUV5, nke bụ ụdị na-arụ ọrụ nke onye na-akwalite lac.Site na ijikọ ntinye IPTG na onye na-akwalite mutant a, ndị nchọpụta nwere ike nweta ọkwa dị elu nke mkpụrụ ndụ ihe nketa.Nke a na-enye ohere maka mmepụta nke nnukwu protein maka ime ka ọ dị ọcha ma ọ bụ ngwa ndị ọzọ na-agbada.
Na mgbakwunye na okwu mkpụrụ ndụ ihe nketa, a na-ejikwa IPTG ugboro ugboro na nyocha nyocha na-acha anụnụ anụnụ/ọcha.Na usoro a, a na-ejikọta mkpụrụ ndụ lacZ na mkpụrụ ndụ ihe nketa nke mmasị, na nje bacteria na-egosipụta nke ọma nke ọma gene fusion ga-emepụta enzyme β-galactosidase na-arụ ọrụ.Mgbe agbakwunyere IPTG yana mkpụrụ chromogenic dị ka X-gal, nje bacteria na-egosipụta mkpụrụ ndụ ihe nketa na-atụgharị na-acha anụnụ anụnụ n'ihi ọrụ β-galactosidase.Nke a na-enye ohere maka njirimara na nhọrọ nke ụdị recombinant nke jikọtara mkpụrụ ndụ ihe nke ọma nke ọma.
Ntinye okwu mkpụrụ ndụ ihe nketa: A na-ejikarị IPTG mee ka ngosipụta nke mkpụrụ ndụ ihe nketa lekwasịrị anya na sistemu nje.Ọ na-eṅomi lactose inducer eke na-ejikọta ya na protein lac repressor, na-egbochi ya igbochi lac operon.Nke a na-enye ohere maka ederede na nkwupụta nke mkpụrụ ndụ ihe nketa chọrọ.
Nkwuputa protein na ime ka ọ dị ọcha: A na-ejikarị ntinye IPTG na-emepụta nnukwu proteins recombinant maka ebumnuche dị iche iche, dị ka ọmụmụ biochemical, mmepụta ọgwụgwọ, ma ọ bụ nyocha nhazi.Site na iji vectors okwu kwesịrị ekwesị na ntinye IPTG, ndị nchọpụta nwere ike nweta ọkwa dị elu nke mmepụta protein ezubere iche na ndị agha nje.
Nleba anya na-acha anụnụ anụnụ / ọcha: A na-ejikarị IPTG na-ejikọta ya na mkpụrụ ndụ lacZ na mkpụrụ chromogenic, dị ka X-gal, maka nyocha nyocha na-acha anụnụ anụnụ / ọcha.A na-ejikọta mkpụrụ ndụ lacZ na mkpụrụ ndụ ihe nketa mmasị, na nje bacteria na-egosipụta nke ọma nke ọma gene fusion ga-emepụta enzyme β-galactosidase na-arụ ọrụ.Mgbe agbakwunyere IPTG na mkpụrụ osisi chromogenic, ụdị recombinant na-egosipụta mkpụrụ ndụ ngwakọta na-acha anụnụ anụnụ, na-enye ohere njirimara na nhọrọ dị mfe.
Ọmụmụ usoro iwu mkpụrụ ndụ ihe nketa: A na-ejikarị ntinye IPTG na nyocha iji mụọ ụkpụrụ nke mkpụrụ ndụ ihe nketa na operons, karịsịa lac operon.Site n'ịgbanwe mkpokọta nke IPTG na nyochaa ngosipụta nke akụrụngwa lac operon, ndị nyocha nwere ike nyochaa usoro nhazi mkpụrụ ndụ ihe nketa yana ọrụ nke ihe dị iche iche ma ọ bụ mmụgharị.
Sistemụ okwu mkpụrụ ndụ ihe nketa: IPTG bụ akụkụ dị oke mkpa n'ọtụtụ sistemụ okwu mkpụrụ ndụ ihe nketa, dị ka sistemụ dabere na T7.N'ime usoro ndị a, a na-ejikarị onye na-akwalite lac na-ebugharị okwu nke T7 RNA polymerase, nke, n'aka nke ya, na-edepụta mkpụrụ ndụ ihe nketa lekwasịrị anya n'okpuru njikwa nke usoro nkwalite T7.A na-eji IPTG mee ka ngosipụta nke T7 RNA polymerase pụta, na-eduga n'ịkwalite nkwupụta mkpụrụ ndụ ihe mgbaru ọsọ.
| Ihe mejupụtara | C9H18O5S |
| Nyocha | 99% |
| Ọdịdị | Ọcha ntụ ntụ |
| CAS Mba. | 367-93-1 |
| Nkwakọ ngwaahịa | Obere na nnukwu |
| Ndụ shelf | afọ 2 |
| Nchekwa | Chekwaa na ebe dị jụụ na nke akọrọ |
| Asambodo | ISO. |
