Kedu ka esi achọpụta mkpokọta kachasị mma?
Maka inducer IPTG (isopropyl-beta-d-thiogalactoside), nke dị elu nke itinye uche, ka mma.Ntinye uche kacha mma na-adabere na ọnọdụ nnwale akọwapụtara na mmetụta nnabata achọrọ.
N'ozuzu, a na-eji ntinye uche nke IPTG na oke nke 0.1-1 mM.Nleta dị ala nwere ike ibelata mmetụta ọjọọ na uto cell ma nwee ike ibelata cytotoxicity n'ihi oke iwepụta protein ndị e lekwasịrị anya.Mmụba dị elu nwere ike ime ka ibu metabolism dị elu nke cell, na-emetụta uto cell na nrụpụta okwu.
Uzo esi ekpebi itinye uche kacha mma nwere ike ịbụ inyocha ọkwa ngosipụta nke protein ebumnuche site na ịme ule nnabata IPTG na mkpokọta dị iche iche.Enwere ike ime ule omenaala pere mpe site na iji ọtụtụ ọnụọgụ IPTG (dịka 0.1 mM, 0.5 mM, 1 mM, wdg) enwere ike nyochaa mmetụta okwu na ntinye dị iche iche site na ịchọpụta ọkwa ngosipụta nke protein ebumnuche (dịka Western Western). ntụpọ ma ọ bụ fluorescence nchọpụta).Dabere na nsonaazụ nnwale ahụ, a na-ahọrọ itinye uche na mmetụta ngosipụta kachasị mma dị ka ntinye kachasị mma.
Na mgbakwunye, ị nwekwara ike na-ezo aka na akwụkwọ ndị dị mkpa ma ọ bụ ahụmahụ nke ụlọ nyocha ndị ọzọ iji ghọta ụdị ịta ahụhụ IPTG a na-ejikarị eme ihe n'okpuru ọnọdụ nnwale ndị yiri ya, wee kwalite ma gbanwee dịka mkpa nyocha.
Ọ dị mkpa iburu n'obi na ntinye kachasị mma nwere ike ịdịgasị iche dabere na usoro okwu dị iche iche, protein ndị e lekwasịrị anya, na ọnọdụ nnwale, n'ihi ya, ọ kacha mma ịkwalite n'otu n'otu.
Oge nzipu: Sep-28-2023