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ONPG CAS: 369-07-3 Onye nrụpụta ọnụahịa

O-nitrophenyl-β-D-galactopyranoside (ONPG) bụ ihe sịntetịt eji na biochemical na molecular biology assays iji tụọ ọrụ nke enzyme β-galactosidase.A na-ejikarị ya eme nyocha iji chọpụta mkpụrụ ndụ ihe nketa na sistemu nje, dị ka Escherichia coli.ONPG bụ ihe na-enweghị ụcha nke β-galactosidase gbawara, na-ebute ntọhapụ nke ogige odo, o-nitrophenol.Enwere ike tụọ agba odo na-emepụta spectrophotometrically, na-enye nha nke ọrụ enzyme na-apụtaghị ìhè. A na-akpọkarị nyocha nke na-eji ONPG dị ka nyocha ONPG na bụ usoro a na-ejikarị eme ihe na nchọpụta ihe ọmụmụ ihe omimi iji chọpụta ọkwa ọkwa nke mkpụrụ ndụ ihe nketa nke lac na-achịkwa. operon na sel nje bacteria.


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Ngwa na Mmetụta

Mmetụta nke ONPG dị ka mkpụrụ bụ nke enzyme β-galactosidase ga-agbaji, na-ebute ntọhapụ nke ngwaahịa edo edo, o-nitrophenol.Enwere ike tụọ mgbanwe agba a n'ụzọ spectrophotometrically, na-enye ohere maka nhazi nke ọrụ β-galactosidase. Ngwa nke ONPG bụ isi na nyocha nke okwu mkpụrụ ndụ ihe nketa na ihe ọmụmụ ihe omimi na nchọpụta microbiology.A na-ejikarị elele ọrụ β-galactosidase dị ka onye nta akụkọ maka ọmụmụ okwu mkpụrụ ndụ ihe nketa, karịsịa na nje bacteria dị ka E. coli.A na-ejikarị mkpụrụ ndụ lacZ, nke na-edekọ β-galactosidase, dị ka ihe nrịbama maka nyocha okwu mkpụrụ ndụ ihe nketa, n'ihi na okwu ya nwere ike ịmalite site na ọnọdụ dị iche iche ma ọ bụ na-achịkwa ya site n'aka ndị na-akwalite ya. Nnwale ONPG na-enye ụzọ dị mma na nke a pụrụ ịdabere na ya iji nyochaa ọkwa nke ọkwa. okwu nke mkpụrụ ndụ ihe nketa site n'ịtụ ọrụ β-galactosidase.A na-eji nyocha a eme ihe n'ọtụtụ ngwa dị iche iche, dị ka ịmụ ọrụ nkwalite, nhazi mkpụrụ ndụ ihe nketa, na mmekọrịta protein-protein.Tụkwasị na nke a, enwere ike iji ya chọpụta kinetics enzyme ma nyochaa mmetụta nke mmụgharị ma ọ bụ ọgwụgwọ na ọrụ enzyme.

Nlereanya ngwaahịa

369-07-3-1
369-07-3-2

Nkwakọ ngwaahịa:

6892-68-8-3

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CAS Mba. 369-07-3
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