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TRIS-Acetate CAS: 6850-28-8 Onye nrụpụta ọnụahịa

TRIS-Acetate, bụ ihe nchekwa a na-ejikarị na nyocha nke ndu na biochemical.Ọ bụ ngwakọta nke Tris base na acetic acid, na-eme ka ngwọta pH-stable nke a na-eji na-achịkwa ma na-ejigide pH nke chọrọ maka ngwa dị iche iche. TRIS-Acetate bara uru karịsịa na DNA na RNA ọmụmụ, dị ka ọ na-enye. gburugburu ebe kwesịrị ekwesị maka ọrụ enzyme, electrophoresis, na gel electrophoresis.Ọ na-enyere aka ịnọgide na-enwe nkwụsi ike na iguzosi ike n'ezi ihe nke nucleic acid n'oge usoro dị iche iche, dị ka DNA sequencing, polymerase chain reaction (PCR), na agarose gel electrophoresis.Na mgbakwunye na nchọpụta nucleic acid, a na-ejikwa TRIS-Acetate na protein dịpụrụ adịpụ na usoro dị ọcha. , mmịpụta protein akpụkpọ ahụ, na nnwale omenala cell.Ikike nchekwa ya dị iche iche na-eme ka ọ bụrụ ngwá ọrụ bara uru n'ọtụtụ ebe nyocha sayensị, na-eme ka ọnọdụ kachasị mma maka mmeghachi omume nke ndu na idowe nkwụsi ike nke enzymes na protein.


Nkọwa ngwaahịa

Mkpado ngwaahịa

Ngwa na Mmetụta

Tris-acetate (TRIS-Acetate) bụ ihe nchekwa a na-ejikarị na nyocha nke ndu na biochemical.Ọ nwere ngwakọta nke tris (hydroxymethyl)aminomethane (Tris) na acetic acid, nke na-arụ ọrụ dị ka pH regulator na stabilizer.PH nke ihe nchekwa TRIS-Acetate na-adịkarị site na 7.4 ruo 8.4.
Mmetụta bụ isi nke TRIS-Acetate bụ ịnọgide na-enwe pH kwụsiri ike, nke dị oké mkpa maka ọtụtụ mmeghachi omume biochemical.Ọ na-arụ ọrụ dị ka ihe nchekwa site na ibelata mgbanwe ọ bụla dị ịrịba ama na pH nke nwere ike ime n'ihi acids ma ọ bụ ntọala agbakwunyere n'oge usoro nnwale.
TRIS-Acetate na-achọta ngwa dị iche iche na bayoloji molecular, biochemistry, na biotechnology:
DNA na RNA Electrophoresis: A na-ejikarị TRIS-Acetate eme ihe dị ka ihe na-agba ọsọ na agarose na polyacrylamide gel electrophoresis.Ọ na-enye gburugburu pH kwụsiri ike n'oge nkewa nke DNA na iberibe RNA dabere na nha ha.
Nyocha protein: A na-eji TRIS-Acetate buffers maka protein electrophoresis, dị ka SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis).Ọ na-eme ka protein kwụsie ike na nkewa n'oge usoro ahụ.
Mmeghachi omume Enzyme: A na-ejikarị ihe nchekwa TRIS-Acetate na nyocha enzyme na ọmụmụ ihe.Ọ na-enye oke pH kachasị mma maka mmeghachi omume enzymatic dị iche iche ma na-enyere aka ịnọgide na-arụ ọrụ enzyme.
Omenala Cell na Tissue: A na-eji ihe nchekwa TRIS-Acetate na mgbasa ozi omenala cell iji nọgide na-enwe pH kwesịrị ekwesị maka uto cell na mmụba.Ọ na-enyere aka ịnọgide na-enwe ọnọdụ physiological chọrọ maka ndụ cell.

Nkwakọ ngwaahịa:

6892-68-8-3

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CAS Mba. 6850-28-8
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