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3-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE CAS:3150-25-2

3-Nitrophenyl-beta-D-galactopyranoside (ONPG) bụ mkpụrụ a na-ejikarị na nyocha enzymatic iji chọpụta ma tụọ ọrụ beta-galactosidase.Mgbe beta-galactosidase dị ma na-arụ ọrụ, ọ na-eme ka ONPG hydrolyzes, na-ahapụ ngwaahịa na-acha odo odo nke a na-akpọ 3-nitrophenol.Enwere ike tụọ ike nke agba edo edo emepụtara n'ụzọ spectrophotometrically, na-enye ohere nha nke ọrụ beta-galactosidase.A na-ejikarị ONPG eme ihe na usoro ihe ọmụmụ ihe omimi na nyocha microbiology, yana na nyocha ụlọ ọgwụ, iji mụọ okwu mkpụrụ ndụ ihe nketa, ọrụ protein, njirimara nje bacteria, na ike cell.

 


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Ngwa na Mmetụta

Nchọpụta ọrụ beta-galactosidase: ONPG na-ejikarị achọpụta ọnụnọ na ọrụ nke beta-galactosidase na ụdị dị iche iche nke ndu, dị ka omenala nje ma ọ bụ cell lysates.Mmepụta nke o-nitrophenol, nke nwere agba odo, nwere ike tụọ ngwa ngwa site na iji spectrophotometer.

Ọmụmụ okwu mkpụrụ ndụ: A na-ejikarị ONPG eme nchọpụta ihe ọmụmụ ihe gbasara mkpụrụ ndụ ihe nketa.Site n'ijikọta onye na-akwalite mkpụrụ ndụ ihe nketa mmasị na mkpụrụ ndụ ihe nketa nke na-etinye beta-galactosidase, ndị nchọpụta nwere ike tụọ ọrụ nke onye nkwalite a site na ịgbakwunye ONPG na ịkọwapụta mmepụta o-nitrophenol.Usoro a, nke a maara dị ka beta-galactosidase reporter assay, na-enye ozi gbasara ọrụ ederede nke mkpụrụ ndụ ihe nketa.

Nchọpụta nje bacteria: Ụfọdụ nje bacteria na-emepụta beta-galactosidase, ebe ndị ọzọ adịghị.Enwere ike iji ONPG yana nyocha biochemical ndị ọzọ iji chọpụta ụdị nje na-adabere n'ikike ha nwere hydrolyze ONPG.A na-ejikarị usoro a na nyocha ụlọ ọgwụ na ụlọ nyocha microbiology.

Nyocha maka ndị na-egbochi enzyme ma ọ bụ ndị na-eme ihe: ONPG nwere ike iji nyochaa ogige ndị na-agbanwe ọrụ beta-galactosidase.Site n'ịtụle ọrụ enzyme na ọnụnọ nke ogige dị iche iche, ndị na-eme nchọpụta nwere ike ịchọpụta ndị nwere ike igbochi ma ọ bụ ndị na-arụ ọrụ nke nwere ike nyochaa ọzọ maka ike ọgwụgwọ ha.

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Ihe mejupụtara C12H15NO8
Nyocha 99%
Ọdịdị Ọchantụ ntụ
CAS Mba. 3150-25-2
Nkwakọ ngwaahịa Obere na nnukwu
Ndụ shelf afọ 2
Nchekwa Chekwaa na ebe dị jụụ na nke akọrọ
Asambodo ISO.

 


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