3,3 ,5,5 -Tetramethylbenzidine dihydrochloride hydrate CAS:207738-08-7

3,3',5,5'-Tetramethylbenzidine dihydrochloride (TMB) bụ ihe a na-ejikarị eme ihe n'ule enzyme-linked immunosorbent assays (ELISAs) na nyocha biochemical ndị ọzọ. Mgbe enzyme horseradish peroxidase (HRP) na-agba TMB ọkụ n'ihu hydrogen peroxide, ọ na-emepụta agba anụnụ anụnụ. A na-eji mgbanwe agba a achọpụta ọnụnọ nke ọgwụ mgbochi HRP ma ọ bụ protein ndị ọzọ, na-egosikarị ọnụnọ nke antigen kpọmkwem na sample. A na-ejikarị spectrophotometer tụọ agba anụnụ anụnụ nke mmeghachi omume ahụ mepụtara, nke na-enye ohere maka nha nke ike nke mgbanwe agba ma na-enye ụzọ iji jikọta ike agba na ọnụọgụ nke molekul ebumnuche dị na sample ahụ. TMB nwere ọtụtụ ngwa na nyocha nchọpụta, nyocha, na mmepe ọgwụ, karịsịa na nchọpụta nke antibodies, hormones, cytokines, na biomolecules ndị ọzọ. Mmetụta ya, nkwụsi ike ya, na ịdị mfe nke ojiji ya na-eme ka ọ bụrụ ngwaọrụ bara uru na ntọala ụlọ nyocha dị iche iche maka nchọpụta na nhazi nke molekul kpọmkwem.