3,3′,5,5′-Tetramethylbenzidine sulfate CAS:54827-18-8

ELISA: A na-ejikarị ya eme ihe dị ka ihe ndabere maka horseradish peroxidase (HRP) na nyocha ELISA. N'ihe gbasara molekul a na-achọ, dị ka antigen ma ọ bụ antibody, enzyme HRP na-akwalite oxidation nke 3,3', 5,5'-Tetramethylbenzidine sulfate, na-eduga n'ịmepụta ngwaahịa na-acha anụnụ anụnụ. Ike nke agba ahụ kwekọrọ kpọmkwem na mkpokọta nke molekul a na-achọ, na-enye ohere maka nyocha ọnụọgụ.

Nnyocha ọrụ enzyme: Enwere ike iji 3,3',5,5'-Tetramethylbenzidine sulfate tụọ ọrụ enzyme. Site n'itinye ihe nlele enzyme na substrate, enwere ike nyochaa mmeghachi omume enzymatic site na mgbanwe na agba. Ọrụ enzyme dị elu na-eduga na agba anụnụ anụnụ siri ike karị, na-eme ka o kwe omume ịchọpụta kinetics enzyme na ọmụmụ ihe mgbochi.

Immunohistochemistry: Enwere ike iji ya mee ihe maka usoro agba na immunohistochemistry (IHC). A na-eji ya dị ka chromogen maka ọgwụ mgbochi mbụ ma ọ bụ nke abụọ nke e ji horseradish peroxidase mara, nke na-ebute mmepe nke nkwụnye acha anụnụ anụnụ a na-ahụ anya na ebe mmekọrịta antigen na antibody na-aga.

Nnyocha Biochemical: 3,3',5,5'-Tetramethylbenzidine sulfate na-achọta ngwa na ọmụmụ ihe biochemical dị iche iche, karịsịa na nchọpụta na nha nke ọrụ enzyme, mmekọrịta protein-protein, na mmekọrịta protein-DNA.