4-Methylumbelliferyl-beta-D-glucopyranoside CAS:18997-57-4

Mmetụta nke 4-Methylumbelliferyl-beta-D-glucopyranoside (MUG) bụ iji mee ihe dị ka ihe ndabere maka enzyme beta-glucosidase. Enzyme a na-agbaji njikọ glucosidic nke MUG, na-ebute mwepụta nke 4-Methylumbelliferone (4-MU). Itinye MUG n'ọrụ bụ isi n'ọhịa nke microbiology, karịsịa maka nchọpụta na ịchọpụta nje bacteria na-emepụta beta-glucosidase. A na-ejikarị MUG eme ihe n'ịchọpụta Escherichia coli (E. coli) na mmiri na ihe nlele nri. E. coli nwere enzyme beta-glucosidase, nke nwere ike hydrolyze MUG ma mepụta akara fluorescent n'ihu ìhè ultraviolet (UV). Njirimara fluorescent nke 4-MU na-enye ohere maka nchọpụta na nhazi dị mfe. Mgbe ejiri hydrolyzed substrate MUG, 4-MU emepụtara na-ewepụta fluorescence na-acha anụnụ anụnụ, na-eme ka nchọpụta nke nje bacteria nwere ọrụ beta-glucosidase dị mfe. A na-ejikarị usoro a eme ihe n'ileba anya gburugburu ebe obibi na nnwale nchekwa nri, ebe ọ na-enye ụzọ dị ngwa na nke dị nro iji chọpụta mmetọ nje bacteria. Na mgbakwunye na ojiji ya na microbiology, enwere ike iji MUG mụọ ọrụ na mgbochi nke beta-glucosidase na nyocha biochemistry na molekul bayọlọji. Ọkụ ya na-eme ka a tụọ kinetics enzyme ma enwere ike iji ya nyochaa maka ndị nwere ike igbochi ma ọ bụ ndị na-eme ka ọrụ beta-glucosidase rụọ ọrụ. N'ozuzu, MUG bụ ngwakọta dị iche iche nke na-achọta ngwa ngwa n'ọhịa nke microbiology, biochemistry, na molekul bayọlọji maka nchọpụta nke ọrụ beta-glucosidase na ịchọpụta nje bacteria ndị na-emepụta enzyme a.