4-NITROPHENYL-ALPHA-D-MANNOPYRANOSIDE CAS:10357-27-4

Ihe ndị dị n'ime enzyme: Enwere ike iji 4NPM mee ihe dị ka ihe e ji eme enzymes dị iche iche, gụnyere glycosidases na enzymes ndị yiri ya. Enzymes ndị a na-ekewa njikọ glycosidic dị n'etiti mannose na 4NPM, na-eme ka a wepụta nitrophenyl moiety. Enwere ike tụọ oke hydrolysis nke substrate site na iji spectrophotometric na-enyocha nnabata nke nitrophenyl otu ewepụtara na ogologo ebili mmiri kpọmkwem. Nke a na-enye ndị nchọpụta ohere inyocha ọrụ enzyme na kinetics.

Nnyocha maka mgbanwe carbohydrate: Site n'iji 4NPM dị ka ihe ndabere, ndị nchọpụta nwere ike ịmụ ọrụ nke enzymes ndị metụtara metabolism carbohydrate, dị ka alpha-mannosidases. Enzymes ndị a na-eme ka njikọ glycosidic dị na ihe ndị nwere mannose, a pụkwara ịtụ ọrụ ha site na ileba anya na mwepụta nke nitrophenyl moiety site na 4NPM.

Ọmụmụ Glycosylation: Enwere ike iji 4NPM mee ihe n'ule iji nyochaa enzymes ndị metụtara usoro glycosylation. Glycosylation bụ usoro nke ijikọ molekul shuga na protein ma ọ bụ molekul ndị ọzọ, ọtụtụ enzymes na-etinyekwa aka na usoro a. Site na iji 4NPM dị ka ihe na-anabata ihe, ndị nchọpụta nwere ike ịtụle mbufe nke ihe shuga na 4NPM site na enzymes kpọmkwem ndị metụtara mmeghachi omume glycosylation.

Nnyocha maka ihe mgbochi enzyme ma ọ bụ ihe na-eme ka ihe rụọ ọrụ: Enwere ike iji 4NPM mee ihe na nyocha nyocha dị elu iji chọpụta ihe ndị na-egbochi ma ọ bụ na-eme ka enzymes ụfọdụ rụọ ọrụ. Site n'inyocha mmetụta nke ihe ndị a na-anwale na hydrolysis ma ọ bụ mgbanwe nke 4NPM site na enzymes ndị a na-elekwasị anya, ndị nchọpụta nwere ike ịchọpụta ihe ndị nwere ike ịgwọ ọrịa ma ọ bụ ihe nyocha kemịkalụ bara uru maka ịmụ ọrụ enzyme.