4-Nitrophenyl beta-D-galactopyranoside CAS:200422-18-0

Mmetụta: ONPG bụ ihe e ji eme ihe kpọmkwem iji chọpụta ọnụnọ na ọrụ nke enzyme β-galactosidase. Mgbe enzyme β-galactosidase dị ma na-arụ ọrụ, ọ na-ekewa ONPG n'ime ngwaahịa abụọ: o-nitrophenol na ihe sitere na galactose. Ntọhapụ nke o-nitrophenol na-ebute mgbanwe agba odo odo, nke enwere ike iji spectrophotometer tụọ.

Ngwa: ONPG nwere ọtụtụ ngwa na nchọpụta bayọlọjik molekul na biochemistry:

Nchọpụta nke ọrụ β-galactosidase: A na-ejikarị ONPG atụ ma tụọ ọrụ nke enzyme β-galactosidase. Enwere ike tụọ ọnụego nke mmepụta o-nitrophenol, nke dabara kpọmkwem na ọrụ enzyme, site na spectrophotometry.

Mpụta na nhazi mkpụrụ ndụ ihe nketa: A na-ejikarị ONPG eme ihe na nnwale metụtara mpụta mkpụrụ ndụ ihe nketa na ọmụmụ nhazi. A na-ejikarị ya eme ihe na nnwale protein fusion, dịka sistemụ mpụta lacZ a na-ejikarị eme ihe, iji mụọ ngosipụta mkpụrụ ndụ ihe nketa n'okpuru njikwa nke ndị na-akwalite ya kpọmkwem. Ọrụ beta-galactosidase nke a tụrụ site na iji ONPG na-enye nghọta n'ọkwa mpụta mkpụrụ ndụ ihe nketa.

Nnyocha maka ọrụ β-galactosidase: Enwere ike iji ONPG dị ka ụzọ nyocha agba na teknụzụ DNA recombinant iji chọpụta ọnụnọ na ọrụ nke mkpụrụ ndụ ihe nketa LacZ, nke na-akọwapụta β-galactosidase. Usoro nyocha a na-enyere aka n'ịchọpụta klọn ndị nwere mkpụrụ ndụ ihe nketa mmasị.

Ọmụmụ gbasara kinetics nke enzyme: ONPG bara uru n'ịmụ gbasara kinetics nke enzyme β-galactosidase. Site n'ịtụle ọnụego nke mmeghachi omume enzyme-substrate na njupụta substrate dị iche iche, ọ ga-ekwe omume ịchọpụta paramita kinetic dị ka Michaelis-Menten constants (Km) na ọnụego mmeghachi omume kachasị elu (Vmax).