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5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide CAS:4264-82-8

5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide bụ ngwakọta a na-eji n'ọmụmụ ihe ọmụmụ biochemical dị iche iche, ọkachasị maka nchọpụta na nhụta ọrụ enzyme.Ọ bụ mkpụrụ nke nwere ike ịmegharị ya site na enzymes kpọmkwem, na-ebute ntọhapụ nke ngwaahịa agba ma ọ bụ fluorescent.

A na-ejikarị ọgwụ a eme ihe na nyocha iji chọpụta ọnụnọ na ọrụ nke enzymes dị ka beta-galactosidase na beta-glucuronidase.Enzymes ndị a na-ekewa ndị acetyl na glucosaminide site na mkpụrụ, na-eduga n'ichebe chromophore na-acha anụnụ anụnụ ma ọ bụ akwụkwọ ndụ akwụkwọ ndụ.

Ọdịdị pụrụ iche nke 5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide na-enye ohere maka nchọpụta dị mfe na ọnụọgụ ọrụ enzyme.Ojiji ya na usoro nnwale dị iche iche, gụnyere histochemistry, immunohistochemistry, na nyocha nke sitere na cell, enyela aka na nghọta ka mma nke ọrụ enzyme.


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Ngwa na Mmetụta

5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide (X-Gluc) bụ mkpụrụ chromogenic nke a na-ejikarị achọpụta ọrụ beta-glucuronidase (GUS).GUS bụ enzyme a na-achọta n'ime ihe dị iche iche, gụnyere nje bacteria, osisi, na mammals.A na-ejikarị X-Gluc eme ihe na nyocha ndị nta akụkọ GUS na nyocha ihe ọmụmụ ihe omimi.

Isi ngwa nke X-Gluc dị na usoro ihe nchacha histochemical, ebe ọ nwere ike iji anya nke uche hụ okwu na ọrụ nke GUS enzyme.Mkpụrụ a na-ekesa mkpụrụ ndụ wee bụrụ hydrolyzed site na GUS, na-ebute n'ichepụta ngwaahịa na-acha anụnụ anụnụ ma ọ bụ ngwaahịa na-adịghị ike.Nke a na-acha anụnụ anụnụ na-enye ndị na-eme nchọpụta aka ịchọpụta na ịchọta ọrụ GUS na sel, anụ ahụ, na ihe niile dị ndụ.

A pụkwara iji X-Gluc mee ihe na ọnụọgụ ọnụọgụ iji tụọ ọrụ enzyme GUS.Ike nke agba anụnụ anụnụ ma ọ bụ ọnụ ọgụgụ nke ngwaahịa etolite nwere ike jikọta ya na ọkwa GUS ma ọ bụ ọrụ enzymatic ya.

Na mgbakwunye, ejirila X-Gluc n'ọrụ na nyocha mkpụrụ ndụ ihe ọkụkụ iji mụọ okwu mkpụrụ ndụ ihe nketa, ọrụ nkwalite na mgbanwe osisi.A na-ejikwa ya na sistemu nje maka cloning na ịchọpụta protein fusion GUS.

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CAS Mba. 4264-82-8
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