HEPES CAS:7365-45-9 Ọnụahịa Onye Mmepụta

HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid) bụ ihe nchekwa bayọlọji nke a na-ejikarị eme nnyocha bayọlọji na kemịkalụ. A maara ya maka ikike ya ịnọgide na-enwe pH kwụsiri ike na mmiri mmiri, na-eme ka ọ dabara adaba maka ojiji dị iche iche gụnyere usoro sel na enzymatic. HEPES bụ ihe mejupụtara zwitterionic, nke pụtara na ọ nwere ebubo dị mma na nke na-adịghị mma na otu ọrụ dị iche iche, nke na-enye ya ohere iguzogide mgbanwe na pH nke mgbakwunye nke asịd ma ọ bụ ntọala kpatara. A na-ejikarị ya eme ihe na omenala sel, nnwale enzyme, ọmụmụ protein, nnwale electrophoresis, na usoro ọgwụ.

Nye iwu ma ọ bụ rịọ maka ọnụahịa

Nkọwa Ngwaahịa

Akara Ngwaahịa

Ngwa na Mmetụta

Nchekwa pH: A na-ejikarị HEPES eme ihe dị ka ihe na-egbochi ihe iji nọgide na-enwe pH kwụsiri ike na mgbasa ozi omenala mkpụrụ ndụ na nyocha bayoloji. Ọ na-arụ ọrụ nke ọma na oke pH physiological nke 6.8 ruo 8.2, na-eme ka ọ dabara adaba maka ịnọgide na-enwe ọnọdụ kacha mma maka usoro bayoloji.

Omenala mkpụrụ ndụ: A na-agbakwụnye HEPES na mgbasa ozi omenaala mkpụrụ ndụ iji mee ka ọkwa pH kwụsie ike, na-enye gburugburu ebe kwesịrị ekwesị maka uto mkpụrụ ndụ ma na-eme ka mkpụrụ ndụ dị ndụ. Ọ na-enyere aka igbochi mgbanwe pH nke nwere ike imetụta omume mkpụrụ ndụ na nsonaazụ nnwale na-adịghị mma.

Nnyocha Enzyme: A na-ejikarị HEPES eme ihe dị ka ihe nchekwa na mmeghachi omume enzymatic n'ihi ikike ya ịnọgide na-enwe pH kpọmkwem. Ọ na-enyere aka n'ịnọgide na-arụ ọrụ na nkwụsi ike enzymatic kacha mma, si otú a na-eme ka izi ezi na ntụkwasị obi nke nnwale enzyme dịkwuo mma.

Ọmụmụ ihe gbasara protein: A na-eji HEPES eme ihe n'ọtụtụ nnwale metụtara protein, gụnyere nhicha protein, nhazi kristal protein, na nyocha nhazi protein. Ọ na-enyere aka ime ka pH kwụsie ike ma nọgide na-enwe mpịachi protein na nkwụsi ike kwesịrị ekwesị n'oge nnwale ndị a.

Electrophoresis: HEPES na-achọta ojiji na usoro electrophoresis gel, dịka SDS-PAGE na agarose gel electrophoresis. A na-eji ya dị ka ihe na-egbochi ihe na ihe na-agba ọsọ, na-enye gburugburu pH kwụsiri ike maka nkewa na nyocha nke biomolecules.

Usoro ọgwụ: A na-eji HEPES eme ihe n'ịhazi ngwaahịa ọgwụ na nke bayoteknoloji. Ọ na-enyere aka ịnọgide na-enwe nkwụsi ike na iguzosi ike n'ezi ihe nke ihe ndị na-arụ ọrụ, na-ahụ na ha dị irè ma na-adịru ogologo oge.

Nkwakọ ngwaahịa:

Ozi Mgbakwunye:

Ihe mejupụtara ya	C8H18N2O4S
Nnwale	99%
Ọdịdị	Ntụ ntụ ọcha
Nọmba CAS	7365-45-9
Nkwakọ ngwaahịa	Obere na nnukwu
Ndụ nchekwa	afọ 2
Nchekwa	Debe n'ebe dị jụụ ma kpọọ nkụ
Asambodo	ISO.