5,5-Dithiobis(2-nitrobenzoic acid) CAS:69-78-3

5,5-Dithiobis (2-nitrobenzoic acid), nke a makwaara dị ka DTNB ma ọ bụ ihe na-eme ka Ellman dị nro, bụ ihe mejupụtara kemịkalụ a na-ejikarị eme nchọpụta kemịkalụ na ọgwụ. Ọ bụ ntụ ntụ kristal odo odo nke a na-agbaze nke ukwuu na mmiri na ihe mgbaze organic.

A na-ejikarị DTNB atụ nha nke otu sulfhydryl (-SH) na protein na peptides. Ngwakọta ahụ na-emeghachi omume na thiols, na-eme ka e nwee ngwakọta disulfide ma wepụta 2-nitro-5-thiobenzoate, nke nwere agba odo nke enwere ike ịtụ spectrophotometric. Site n'ịtụle ike nke agba odo ahụ, ọ ga-ekwe omume ịtụle mkpokọta thiols na ihe nlele. A na-ejikarị usoro a amụ mmekọrịta protein na protein, mpịachi protein, na mmeghachi omume enzymatic nke gụnyere otu thiol.

Na mgbakwunye na nhazi thiol, ejirila DTNB mee ihe n'ọrụ ndị ọzọ, dịka inyocha ọnọdụ redox nke mkpụrụ ndụ, ịmụ nkwụsi ike nke ihe mejupụtara ígwè na sọlfọ, na ịtụ ọrụ nke enzymes dị iche iche.

Nye iwu ma ọ bụ rịọ maka ọnụahịa

Nkọwa Ngwaahịa

Akara Ngwaahịa

Ngwa na Mmetụta

Ịtụle Thiol: A na-ejikarị DTNB enyocha mkpokọta thiols na ihe atụ protein. Ozi a dị mkpa maka ịmụ mmekọrịta protein na protein, mpịachi protein, na mmeghachi omume enzymatic metụtara otu thiol. Nzaghachi agba nke DTNB na-enye ohere maka ịtụle thiols site na nha spectrophotometric.

Nnyocha nke Redox State: Ejirila DTNB nyochaa ọnọdụ redox n'ime mkpụrụ ndụ, anụ ahụ, ma ọ bụ ihe atụ bayọlọji. Site n'ịtụle mgbanwe na mkpokọta thiol, ndị nchọpụta nwere ike inweta nghọta n'ime nrụgide oxidative, ọrụ antioxidant, na ụzọ mgbaàmà redox.

Nnyocha Enzyme: Mmeghachi omume pụrụ iche nke DTNB na thiols na-eme ka ọ bụrụ ngwa ọrụ bara uru maka nnwale enzyme. Ọtụtụ enzymes, dị ka glutathione-S-transferases, cystathionine-γ-lyase, na thioredoxin reductase, dabere na thiol ma enwere ike ịtụle ma ọ bụ nwalee ya site na iji DTNB.

Nnyocha Nkwụsi Ike nke Ngwakọta Metal-Solfur: A na-eji DTNB enyocha nkwụsi ike na mmeghachi omume nke ihe mgbagwoju anya metal-sulfur na metalloproteins. Ngwakọta ahụ nwere ike ịchọpụta ma tụọ ọnụnọ nke thiols metụtara na chelation nke ion metal, na-enye ozi dị mkpa gbasara mmekọrịta metal-sulfur na kemistri nhazi.

Nkwakọ ngwaahịa:

Ozi Mgbakwunye:

Ihe mejupụtara ya	C14H8N2O8S2
Nnwale	99%
Ọdịdị	Isi odo odo siri ike
Nọmba CAS	69-78-3
Nkwakọ ngwaahịa	Obere na nnukwu
Ndụ nchekwa	afọ 2
Nchekwa	Debe n'ebe dị jụụ ma kpọọ nkụ
Asambodo	ISO.