8-Anilino-1-naphthalenesulfonic acid nnu ammonium CAS:28836-03-5

Ihe ngosi pH: Ihe mejupụtara ya na-agbanwe n'ọkwa kachasị elu nke ikuku fluorescence ya dabere na pH nke ihe ngwọta ọ dị na ya. Njirimara a na-eme ka ọ baa uru dị ka ihe ngosi pH n'ọtụtụ ngwa, dị ka inyocha mgbanwe pH na ihe nlele bayoloji ma ọ bụ ịmụ mmeghachi omume enzymatic.

Nyocha nhazi protein: 8-Anilino-1-naphthalenesulfonic acid nnu ammonium nwere ike ijikọ na protein ma gbanwee ike fluorescence ma ọ bụ njirimara spectral dabere na nhazi protein ahụ. Nke a na-eme ka ọ bụrụ ngwaọrụ bara uru maka ịmụ mpịachi protein, denaturation, na mgbanwe nhazi.

Mmekọrịta Protein na ligand: Enwere ike iji ihe mejupụtara ya mụọ mmekọrịta protein na ligand. Site na ijikọ na protein ma na-agbanwe fluorescence mgbe njikọ ligand gasịrị, ọ na-enye ndị nchọpụta ohere inyocha njikọ njikọ, kinetics, na usoro nke ọrụ nke ligand dị iche iche.

Nchọpụta Ion: Ejirila nnu ammonium 8-Anilino-1-naphthalenesulfonic acid chọpụta ma tụọ ọnụọgụ ion ígwè dị iche iche, dị ka calcium, magnesium, na zinc. Enwere ike ịgbanwe àgwà fluorescence nke ihe mejupụtara ya site na ọnụnọ nke ion ndị a, na-enye usoro dị nro na nke nhọrọ maka nchọpụta ha.

Nlekota gburugburu ebe obibi: Ejirila ihe mejupụtara ya mee ihe maka nlekota gburugburu ebe obibi, dịka ịchọpụta ihe ndị na-emerụ emerụ na nlekota mgbanwe na mma mmiri. Njirimara fluorescence ya na-eme ka a mata ihe ndị na-emerụ emerụ ngwa ngwa na nke dị nro na ihe nlele mmiri.

Kemịstrị nyocha: A na-ejikwa nnu ammonium 8-Anilino-1-naphthalenesulfonic acid eme ihe n'ọtụtụ usoro nyocha, dịka fluorescence spectroscopy na high-performance liquid chromatography (HPLC), maka nha na njirimara nke ihe mejupụtara na ngwakọta dị mgbagwoju anya.