8-Anilino-1-naphthalenesulfonic acid CAS:82-76-8

8-Anilino-1-naphthalenesulfonic acid, nke a makwaara dị ka ANS, bụ agba fluorescent nke a na-ejikarị eme nchọpụta biochemical na biophysical. Ọ bụ nke otu ihe ndị dị ndụ a maara dị ka sulfonic acid nke dabere na aniline. A maara ANS maka ikike ya ijikọ na mpaghara hydrophobic ma ọ bụ ebe hydrophobic ekpughere na protein na biomolecules ndị ọzọ.

ANS na-enwu enwu mgbe ọ na-ejikọta na mpaghara hydrophobic, nke na-eme ka ọ bụrụ ihe nyocha bara uru maka ịmụ mgbanwe nhazi protein, mmekọrịta protein na protein, na usoro mpịachi/mmepe protein. Njikọ nke ANS na mpaghara hydrophobic na-eduga na mgbanwe anụnụ anụnụ na spectrum emission, nke enwere ike inyocha site na iji fluorescence spectroscopy.

A na-ejikarị ANS eme ihe n'ọtụtụ usoro nyocha dị iche iche, gụnyere fluorescence polarization, fluorescence resonance energy transfer (FRET), na thermal stability assays. Ejirila ya mụọ nhazi na nkwụsi ike nke protein, mmekọrịta protein na ligand, na usoro nchịkọta protein.

Nye iwu ma ọ bụ rịọ maka ọnụahịa

Nkọwa Ngwaahịa

Akara Ngwaahịa

Ngwa na Mmetụta

Nchọpụta Fluorescent: A na-ejikarị ANS eme ihe dị ka nyocha fluorescent iji mụọ mgbanwe nhazi protein, usoro mpịachi/mgbapụta protein, na mmekọrịta protein na ligand. Ọ na-ejikọ na mpaghara hydrophobic ma ọ bụ ebe hydrophobic ekpughere na protein, na-ebute mwepụ fluorescent nke enwere ike inyocha site na iji fluorescence spectroscopy.

Nchọpụta Hydrophobicity: ANS na-enwe mmetụta maka mgbanwe na hydrophobicity nke microenvironment. Enwere ike iji ya chọpụta ma tụọ surfactants, ihe nhicha, na ihe ndị ọzọ hydrophobious.

Nnyocha nkwụsi ike nke protein: A na-ejikarị ANS eme ihe na nyocha nkwụsi ike nke okpomọkụ iji chọpụta nkwụsi ike nke protein. Site na ileba anya na ike fluorescence nke protein ndị ANS na-ejikọta n'okpuru okpomọkụ dị iche iche, ọ ga-ekwe omume ịchọpụta njirimara denaturation okpomọkụ na okpomọkụ agbaze (Tm) nke protein ahụ.

Ọmụmụ ihe gbasara nchịkọta protein: Enwere ike iji ANS mụọ usoro nchịkọta protein, dịka nhazi nke amyloid fibrils. Agba ahụ na-enwe mmetụta na mpaghara hydrophobic a na-ekpughe na mkpokọta protein, na-eme ka ọ bụrụ ngwaọrụ bara uru maka inyocha ọganihu na kinetics nke nchịkọta.

Nkọwa nke mmekọrịta protein na protein: Ejirila ANS nyochaa mmekọrịta protein na protein. Mgbe protein na-emekọrịta ihe, enwere ike inwe mgbanwe na hydrophobicity ha, nke enwere ike iji ANS fluorescence nyochaa.

Nchọpụta na Mmepe Ọgwụ: Enwere ike iji ANS mee ihe n'inyocha ndị nwere ike ịṅụ ọgwụ. Ọ nwere ike inyere aka inyocha njikọ na mmekọrịta nke obere molekul na protein ndị a na-achọ, na-enye ndị nchọpụta ohere ịchọpụta ihe ndị nwere ike ịgbanwe ọrụ protein.

Nkwakọ ngwaahịa:

Ozi Mgbakwunye:

Ihe mejupụtara ya	C16H13NO3S
Nnwale	99%
Ọdịdị	Ntụ ntụ akwụkwọ ndụ gbara ọchịchịrị
Nọmba CAS	82-76-8
Nkwakọ ngwaahịa	Obere na nnukwu
Ndụ nchekwa	afọ 2
Nchekwa	Debe n'ebe dị jụụ ma kpọọ nkụ
Asambodo	ISO.