DEPC CAS:1609-47-8 Ọnụahịa Onye Mmepụta

Diethyl pyrocarbonate (DEPC) bụ kemịkalụ a na-ejikarị eme ihe n'ụlọ nyocha bayọlọjik molekul. Ọ bụ mmiri doro anya na enweghị agba nke na-emeghachi omume nke ukwuu na ngwakọta nucleophilic dị ka amines na thiols. A na-ejikarị DEPC eme ihe iji gbochie RNases (ribonucleases), enzymes nke nwere ike imebi RNA, site na ịgbanwe ebe ha na-arụ ọrụ. Ọ na-emezu nke a site na imeghachi omume na ihe fọdụrụ na histidine dị na enzyme, nke dị mkpa maka ọrụ ya. Nke a na-eme DEPC ka ọ bụrụ ngwa bara uru na nnwale metụtara RNA ma na-ahụ na nchekwa nke iguzosi ike n'ezi ihe RNA. Na mgbakwunye na njirimara RNase na-egbochi ọrụ ya, DEPC nwekwara ike ịgbanwe biomolecules ndị ọzọ, dị ka protein na DNA, n'ihi mmeghachi omume ya na otu nucleophilic. Ekwesịrị ịkpachara anya mgbe ị na-ejikwa DEPC, ebe ọ bụ na ọ na-egbu egbu ma nwee ike ịgbanwe agbanwe.

Nye iwu ma ọ bụ rịọ maka ọnụahịa

Nkọwa Ngwaahịa

Akara Ngwaahịa

Ngwa na Mmetụta

Mkpọchi nke RNases: Isi ihe mere DEPC ji eme ihe bụ ịkwụsị RNases, bụ́ enzymes ndị nwere ike imebi RNA. DEPC na-emeghachi omume na ihe fọdụrụ na histidine na RNases, na-agbanwe ebe ha na-arụ ọrụ ma na-eme ka ha ghara ịrụ ọrụ. Nke a na-eme ka e nwee nchekwa nke iguzosi ike n'ezi ihe RNA n'oge nnwale dịka mwepụ RNA, ntụgharị azụ, na usoro RNA.

Nchekwa nke ihe nlele RNA: A na-ejikarị ọgwụgwọ DEPC eme ihe na ngwa ụlọ nyocha, dị ka tubes microcentrifuge, tip pipette, na mmiri ejiri mee nnwale RNA. Nke a na-enyere aka igbochi RNases ọ bụla fọdụrụnụ ma gbochie mmebi RNA n'oge njikwa na nchekwa ihe nlele.

Nkwadebe ihe na-adịghị RNAase: Enwere ike iji DEPC gwọọ ihe ngwọta ma ọ bụ ihe ndị na-adịghị RNAase, dị ka ihe nchekwa, ihe ngwọta Tris-EDTA (TE), ma ọ bụ mmiri eji eme ihe na nnwale bayọlọjik molekul. A pụrụ iji ihe ngwọta ndị a a gwọrọ n'enweghị nsogbu iji belata mbibi RNA.

Mgbanwe DNA: DEPC nwekwara ike imeghachi omume na otu amino dị na molekul DNA, nke na-ebute mgbanwe carbethylated nke ntọala DNA. Enwere ike iji nke a mee ihe n'ọtụtụ ọrụ, dịka mmeghachi omume usoro DNA ma ọ bụ ịgbanwe DNA maka ọmụmụ njikọ.

Nkwakọ ngwaahịa:

Ozi Mgbakwunye:

Ihe mejupụtara ya	C6H10O5
Nnwale	99%
Ọdịdị	Mmiri mmiri na-enweghị agba
Nọmba CAS	1609-47-8
Nkwakọ ngwaahịa	Obere na nnukwu
Ndụ nchekwa	afọ 2
Nchekwa	Debe n'ebe dị jụụ ma kpọọ nkụ
Asambodo	ISO.