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N, N-Bis (2-hydroxyethyl) -2-aminoethanesulfonic acid sodium nnu CAS: 66992-27-6
N, N-Bis (2-hydroxyethyl) -2-aminoethanesulfonic acid sodium nnu, nke a makwaara dị ka HEPES sodium salt, bụ ngwakọta kemịkalụ nke a na-ejikarị dị ka onye na-ebufe pH na ụlọ nyocha nke ndu na kemịkal.Ọ na-enyere aka ịnọgide na-enwe pH kwụsiri ike, na-eme ka ọ dị mma maka ngwa dị ka omenala cell, nyocha enzyme, ọmụmụ protein, electrophoresis, na usoro ọgwụ.HEPES sodium nnu na-eme ka ọnọdụ dị mma maka usoro ndu ma kwalite izi ezi na ntụkwasị obi nke nsonaazụ nnwale.
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S-Butyrylthiocholine iodide CAS: 1866-16-6
S-Butyrylthiocholine iodide bụ ngwakọta kemịkalụ a na-ejikarị na nyocha biochemical na enzymatic.Ọ bụ mkpụrụ maka enzyme butyrylcholinesterase (BChE) ma jiri ya tụọ ọrụ ya.
Mgbe S-Butyrylthiocholine iodide bụ hydrolyzed site BhE, ọ na-emepụta thiocholine na butyric acid dị ka ngwaahịa.Enwere ike tụọ ntọhapụ nke thiocholine site na iji nyocha spectrophotometric ma ọ bụ fluorometric, na-enye ohere maka ọnụọgụ ọrụ BchE.
A na-ejikarị S-Butyrylthiocholine iodide eme ihe na ụlọ ọgwụ na nyocha nyocha iji nyochaa ọrụ BhE na nlele dịka plasma ọbara ma ọ bụ anụ ahụ.Enwere ike iji ya nyochaa ọrụ enzymatic nke BhE na ọrụ ya na usoro ndu dị iche iche, yana nyocha na nlekota nke ụfọdụ ọnọdụ ahụike.
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ABTS (2,2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) nnu diammonium) CAS: 30931-67-0
Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate), nke a na-akpọkarị ABTS, bụ mkpụrụ chromogenic nke a na-ejikarị na nyocha biochemical, karịsịa na ngalaba nke enzymology.Ọ bụ ngwakọta sịntetik nke a na-eji tụọ ọrụ nke enzymes dị iche iche, gụnyere peroxidases na oxidases.
ABTS enweghị agba n'ụdị oxidized mana ọ na-atụgharị na-acha anụnụ anụnụ-acha ndụ ndụ mgbe enzyme oxidized na ọnụnọ nke hydrogen peroxide ma ọ bụ oxygen molecular.Mgbanwe agba a bụ n'ihi nguzobe nke radical cation, nke na-etinye ìhè n'ụdị a na-ahụ anya.
Mmeghachi omume n'etiti ABTS na enzyme na-emepụta ngwaahịa nwere agba nke nwere ike tụọ spectrophotometrically.Ike nke agba na-adaba na ọrụ enzymatic, na-enye ndị na-eme nchọpụta ohere nyochaa ọnụọgụ enzyme kinetics, mgbochi enzyme, ma ọ bụ enzyme-substrate mmekọrịta.
ABTS nwere ọtụtụ ngwa dị iche iche na mpaghara dị iche iche, gụnyere nyocha ụlọ ọgwụ, nyocha ọgwụ, na sayensị nri.Ọ na-enwe mmetụta nke ukwuu ma na-enye oke ike dị ukwuu, na-eme ka ọ bụrụ nhọrọ a ma ama maka ọtụtụ nyocha biochemical.
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4-NITROPHENYLΑ-D-MALTOHEXAOSIDE CAS:74173-30-1
4-Nitrophenyl α-D-maltohexaoside bụ ngwakọta nke klaasị nke njikọ α-glycosidic.Ọ bụ mpụta nke maltose, nke bụ disaccharide nke mejupụtara nkeji glucose abụọ.N'ime ogige a, a na-eji ihe nitrophenyl dochie otu hydroxyl nke nkeji glucose mbụ.
A na-ejikarị ogige a eme ihe dị ka mkpụrụ n'ime nyocha enzymatic iji mụọ ọrụ nke enzymes dị iche iche, karịsịa ndị na-etinye aka na metabolism carbohydrate.Otu nitrophenyl na-enye ohere maka nchọpụta dị mfe na ọnụọgụ nke mmeghachi omume enzymatic site na ịlele nnabata ma ọ bụ fluorescence nke ngwaahịa gbawara agbawa.
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PIPES CAS: 5625-37-6 Onye nrụpụta ọnụahịa
PIPES (piperazine-1,4-bisethanesulfonic acid) bụ ihe nkpuchi zwitterionic a na-ejikarị na nyocha nke ndu na biochemical.Ọ bụ ihe nchekwa pH dị irè nke nwere ikike dị elu maka idowe ọnọdụ pH kwụsiri ike na pH nke 6.1 ruo 7.5.PIPES nwere obere nnyonye anya na biomolecules ma dabara adaba maka nyocha dabere na ọnọdụ okpomọkụ.A na-ejikarị ya eme ihe na usoro electrophoresis gel na usoro ọgwụ dị ka onye na-eme ka ahụ sie ike.N'ozuzu, PIPES bụ ngwakọta na-ejikarị eme ihe na ntọala nnwale dị iche iche.
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3,3′,5,5′-Tetramethylbenzidine CAS:207738-08-7
3,3′,5,5′-Tetramethylbenzidine, nke a makwaara dị ka TMB, bụ ngwakọta kemịkalụ nke a na-ejikarị dị ka mkpụrụ chromogenic na nyocha immunosorbent jikọtara enzyme (ELISA) na nyocha biochemical ndị ọzọ.A na-ejikarị achọpụta ma chọpụta ọnụnọ nke enzymes dị ka horseradish peroxidase (HRP) na ụdị dị iche iche nke ndu.TMB na-enwe mgbanwe agba site na enweghị agba gaa na-acha anụnụ anụnụ na ọnụnọ nke enzymes ndị a.Mgbe nke ahụ gasịrị, enwere ike ịkwụsị mmeghachi omume ahụ site n'ịgbakwunye acid nke na-agbanwe agba anụnụ anụnụ na agba odo ikpeazụ.Ike nke agba odo na-adaba na ọnụọgụ nke enzyme dị ugbu a, na-enye ohere maka ọnụọgụ.
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APS-5 CAS: 193884-53-6 Onye nrụpụta ọnụahịa
(4-Chlorophenyl) thio-methanol 1- (dihydrogen phosphate) nnu disodium (1: 2) bụ ngwakọta kemịkalụ nke sitere na klaasị nke acridine.Ọ nwere sistemụ mgbanaka 10-methylacridine nwere otu thioether jikọtara na ọnọdụ 4-chlorophenyl.Ngwakọta ahụ nwekwara otu methanol na otu phosphate abụọ nke ion sodium na-ewepụ nke ọma.
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5-Bromo-4-chloro-3-indolyl phosphate disodium nnu CAS: 102185-33-1
5-Bromo-4-chloro-3-indolyl phosphate disodium salt (BCIP) bụ ngwakọta kemịkalụ nke a na-ejikarị na usoro ihe ọmụmụ ihe na ngwa biochemistry.Ọ bụ mkpụrụ chromogenic maka enzymes alkaline phosphatase.
A na-ejikarị BCIP na njikọ nitroblue tetrazolium (NBT) dị ka mkpụrụ iji chọpụta ọrụ alkaline phosphatase.Mgbe BCIP dephosphorylated site na alkaline phosphatase, ụdị nke na-acha anụnụ anụnụ, na-enye ohere maka ịhụta ọnụnọ ma ọ bụ ọrụ enzyme.
Ngwakọta a bara uru karịsịa na ngwa ndị dị ka immunohistochemistry, in situ hybridization, na enzyme-linked immunosorbent assays (ELISAs) iji chọpụta ọnụnọ ma ọ bụ nhazi nke biomolecules ma ọ bụ nucleic acid.Ọgba aghara na-acha anụnụ anụnụ nke BCIP hibere na-enye mgbama a na-ahụ anya nke na-enyere aka n'ịchọpụta na nyocha nke ụmụ irighiri ihe ezubere iche na nlele nnwale.
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Nnu nnu sodium CAS: 139-41-3 Onye nrụpụta ọnụahịa
N, N-Bis (2-hydroxyethyl) glycine sodium nnu bụ ngwakọta kemịkalụ ejiri dị ka onye na-echekwa ihe na ngwa biochemical dị iche iche na biophysical.Ọ na-enyere aka ịnọgide na-enwe ọkwa pH kwụsiri ike na ọnọdụ nnwale, na-eme ka ọ baa uru na ọmụmụ enzyme, nchọpụta protein, omenala cell, na usoro nkwụsị nke Western.
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4-Aminophthalhydrazide AMPPD CAS: 3682-14-2
4-Aminophthalhydrazide, nke a makwaara dị ka 4-APhH, bụ ngwakọta kemịkalụ nwere usoro molekụla C8H8N2O.Ọ bụ nke klaasị nke ogige hydrazide ma sitere na phthalic acid.
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N-Acetyl-L-cysteine CAS: 616-91-1
N-Acetyl-L-cysteine (NAC) bụ ụdị nke amino acid cysteine gbanwetụrụ.Ọ na-enye isi iyi nke cysteine ma nwee ike ịtụgharị ngwa ngwa na tripeptide glutathione, antioxidant siri ike n'ime ahụ.A maara NAC maka akụrụngwa antioxidant na mucolytic, na-eme ka ọ baa uru na ngwa ahụike dị iche iche.
Dị ka antioxidant, NAC na-enyere aka ichebe mkpụrụ ndụ site na mmebi nke radicals free, ụdị oxygen na-emeghachi omume, na nsị.Ọ na-akwadokwa njikọ glutathione, nke na-arụ ọrụ dị oke mkpa na usoro nsị nke anụ ahụ yana idowe usoro ahụ ji alụso ọrịa ọgụ dị mma.
A mụọla NAC maka uru ọ nwere na ahụike iku ume, ọkachasị ndị nwere ọnọdụ dịka bronchitis na-adịghị ala ala, COPD, na cystic fibrosis.A na-ejikarị ya eme ihe dị ka ihe na-atụ anya iji nyere aka dị nro na ịtọpụ imi, na-eme ka ọ dịkwuo mfe ikpochapụ okporo ụzọ ikuku.
Ọzọkwa, NAC egosila nkwa n'ịkwado ahụike imeju site n'inyere aka n'iwepụ ihe ndị na-egbu egbu, dị ka acetaminophen, ihe na-ebelata mgbu.O nwekwara ike inwe mmetụta nchebe megide mmebi imeju nke ịṅụ mmanya na-aba n'anya kpatara.
Na mgbakwunye na akụrụngwa nkwado nke antioxidant na iku ume, NAC enyochala maka uru ọ nwere na ahụike uche.Ụfọdụ nnyocha na-egosi na ọ nwere ike inwe mmetụta dị mma na nsogbu ọnọdụ uche, dị ka ịda mbà n'obi na nsogbu mgbagwoju anya (OCD).
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Acetylthiocholine iodide CAS: 1866-15-5
Acetylthiocholine iodide bụ ngwakọta kemịkalụ nke a na-ejikarị dị ka mkpụrụ na nyocha enzyme iji tụọ ọrụ nke enzyme acetylcholinesterase (AChE).AChE bụ enzyme nke na-eme ka neurotransmitter acetylcholine, nzọụkwụ dị oke mkpa n'ịkwụsị nnyefe mgbaàmà n'etiti mkpụrụ ndụ akwara.
Mgbe AChE na-eme acetylthiocholine iodide, a na-ewepụ otu acetyl, na-eme ka e nwee ion thiocholine na acetate.Thiocholine na-emeghachi omume na reagent na-enweghị ụcha akpọrọ DTNB (5,5'-dithiobis(2-nitrobenzoic acid)) iji mepụta ogige na-acha odo odo nke a na-akpọ 5-thio-2-nitrobenzoate, nke enwere ike tụọ ya na spectrophotometrically.Ọnụego mmepe nke agba na-adaba na ọrụ AChE na nlele.
