Ọkpọkọ CAS:5625-37-6 Ọnụahịa Onye Mmepụta

PIPES (piperazine-1,4-bisenatesulfonic acid) bụ ihe na-eme ka zwitterionic buffering bụrụ ihe a na-ejikarị eme nnyocha gbasara ihe ndị dị ndụ na ihe ndị dị ndụ. O nwere ọtụtụ ihe dị mkpa na ojiji, gụnyere:

Ihe na-eme ka pH bufee: PIPES bụ ihe na-eme ka pH dị mma nke na-enyere aka idobe oke pH kwụsiri ike na nnwale dị iche iche nke bayoloji. A na-ejikarị ya eme ihe na mgbasa ozi ọdịbendị sel, nnwale enzyme, na ngwa bayoloji molekul.

Ike nchekwa dị elu: PIPES nwere ikike nchekwa dị mma n'ime oke pH nke 6.1 ruo 7.5, nke na-eme ka ọ dabara adaba maka idobe ọnọdụ pH kwụsiri ike n'ọtụtụ sistemụ bayoloji.

Mmekọrịta dị nta na biomolecules: A maara PIPES maka obere nnyonye anya ya na usoro biochemical yana obere njikọ ya na protein na enzymes, na-eme ka ọ dị mma maka ịnọgide na-enwe iguzosi ike n'ezi ihe na ọrụ nke biomolecules.

Dabara adaba maka nnwale dabere na okpomọkụ: PIPES nwere ike idobe ihe nchekwa ya n'ọtụtụ okpomọkụ, gụnyere ma okpomọkụ anụ ahụ ma nke dị elu. Nke a na-eme ka ọ dabara adaba maka nnwale ndị chọrọ nkwụsi ike na nkenke n'ọnọdụ okpomọkụ dị iche iche.

Ngwa Electrophoresis: A na-ejikarị PIPES eme ihe dị ka ihe nchekwa na usoro electrophoresis gel, dị ka RNA ma ọ bụ DNA agarose gel electrophoresis, n'ihi obere mmịpụta UV na ikike conductivity dị elu.

Usoro ọgwụ: A na-ejikwa PIPES eme ihe n'usoro ọgwụ dị ka ihe na-egbochi ihe, na-enye nkwụsi ike ma na-ejigide pH kacha mma maka irè ọgwụ.