Fluorescein Isothiocyante CAS:3326-32-7

Fluorescein isothiocyanate (FITC) bụ agba fluorescein a na-ejikarị eme nchọpụta biomedical na ngwa nchọpụta. Ọ bụ ihe sitere na fluorescein nke agbanweela ka ọ nwee otu isothiocyanate, nke na-enye ya ohere ijikọ na protein, ọgwụ mgbochi, na biomolecules ndị ọzọ.

FITC na-ewepụta ọkụ na-acha akwụkwọ ndụ ndụ mgbe ìhè nke ogologo ebili mmiri kwesịrị ekwesị kpaliri ya, nke na-abụkarị ihe dị ka nm 488. Enwere ike ịchọpụta ma tụọ ọkụ a site na iji usoro onyonyo dị iche iche, dị ka microscopy fluorescence, flow cytometry, na fluorescence spectroscopy.

Site n'ijikọta FITC na biomolecules ndị nwere mmasị, ndị nchọpụta nwere ike ịchọpụta ma hụ nkesa, ebe a na-eme ya, na mmekọrịta nke biomolecules ndị a n'ime sel, anụ ahụ, ma ọ bụ ihe atụ bayọlọji. Dịka ọmụmaatụ, enwere ike iji ọgwụ mgbochi FITC nke ejiri akara chọpụta protein ma ọ bụ antigens kpọmkwem na nnwale immunofluorescence ma ọ bụ iji nyochaa ọnụọgụgụ mkpụrụ ndụ na nnwale cytometry flow.

Nye iwu ma ọ bụ rịọ maka ọnụahịa

Nkọwa Ngwaahịa

Akara Ngwaahịa

Ngwa na Mmetụta

Njirimara nke ọkụ: FITC nwere nnukwu mmepụta nke quantum ma na-ewepụta ọkụ akwụkwọ ndụ akwụkwọ ndụ na-egbuke egbuke mgbe ọ na-akpali akpali na ihe dị ka 488 nm. Njirimara ọkụ ya na-eme ka ọ dị mma maka nchọpụta site na iji usoro onyonyo dị iche iche, dị ka microscopy fluorescence, flow cytometry, na fluorescence spectroscopy.

Njikọta Covalent: FITC nwere otu isothiocyanate nke na-enye ya ohere ijikọ na otu amino dị na protein, ọgwụ mgbochi, na biomolecules ndị ọzọ. Njikọta covalent a na-eme ka akara siri ike na nke a kapịrị ọnụ nke molecules ebumnuche, na-eme ka nchọpụta na ịhụ ha anya.

Immunofluorescence: A na-ejikarị ọgwụ mgbochi FITC akpọrọ aha na nnwale immunofluorescence iji chọpụta antigens ma ọ bụ protein ụfọdụ na mkpụrụ ndụ na anụ ahụ. Fluorescence akwụkwọ ndụ akwụkwọ ndụ na-egbuke egbuke nke FITC na-ewepụta na-enyere ndị nchọpụta aka ịchọta ma hụ nkesa nke mkpụrụ ndụ ihe nketa a na-elekwasị anya n'ime ihe atụ bayọlọji.

Sistemụ mmiri: A na-eji ọgwụ mgbochi FITC ma ọ bụ biomolecules ndị ọzọ nwere njikọ fluorochrome eme ihe nke ukwuu na cytometry mmiri iji nyochaa ma hazie mkpụrụ ndụ dabere na ihe nrịbama elu ha ma ọ bụ ihe ndị dị n'ime sel. FITC dakọtara na laser 488 nm nke a na-ejikarị eme ihe na ngwaọrụ cytometry mmiri.

Ọmụmụ mmekọrịta protein: Enwere ike iji FITC mee ihe na ọmụmụ mmekọrịta protein na protein iji chọpụta njikọ na mmekọrịta nke protein. Site n'ịkpọ aha protein ụfọdụ na FITC, ndị nchọpụta nwere ike inyocha mmekọrịta ha site na iji usoro dị ka fluorescence resonance energy transfer (FRET) ma ọ bụ fluorescence polarization.

Nlekota Biomolecule: Enwere ike iji FITC chọpụta ma soro mmegharị nke biomolecules n'ime mkpụrụ ndụ ma ọ bụ anụ ahụ dị ndụ. Site n'itinye akara na FITC maka molecules ndị na-adọrọ mmasị, ndị nchọpụta nwere ike ịmụ banyere ebe ha dị, ike ha, na ụkpụrụ ịzụ ahịa ha n'oge a na-eji fluorescence microscopy.

Ngwa nchọpụta ọrịa: A na-eji ihe nyocha ma ọ bụ ọgwụ mgbochi nke FITC nwere akara na nchọpụta ọrịa maka ọtụtụ ojiji, gụnyere ịchọpụta antigens kpọmkwem, ihe ndị na-efe efe, ma ọ bụ biomarkers na ihe nlele ndị ọrịa. Ihe nyocha fluorescent ndị a na-enye nchọpụta dị nro na nke a kapịrị ọnụ, na-enye ohere maka nchọpụta ngwa ngwa na nke ziri ezi.

Nkwakọ ngwaahịa:

Ozi Mgbakwunye:

Ihe mejupụtara ya	C21H11NO5S
Nnwale	99%
Ọdịdị	Ntụ ntụ odo
Nọmba CAS	3326-32-7
Nkwakọ ngwaahịa	Obere na nnukwu
Ndụ nchekwa	afọ 2
Nchekwa	Debe n'ebe dị jụụ ma kpọọ nkụ
Asambodo	ISO.