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FLUORESCEIN MONO-BETA-D- GALACTOPYRANOSIDE CAS: 102286-67-9

Fluorescein mono-beta-D-galactopyranoside, nke a makwaara dị ka FMG, bụ ngwakọta fluorescent nke a na-ejikarị dị ka mkpụrụ n'ime ụdị biochemical dị iche iche na nyocha usoro ndụ cell.A na-enweta ya site na methyl-beta-D-galactopyranoside site na ijikọta ya na fluorescein molecule.FMG na-eji ya amụ ọrụ beta-galactosidase, enzyme nke na-eme ka hydrolysis nke lactose banye galactose na glucose.Site n'iji FMG dị ka mkpụrụ, ndị nchọpụta nwere ike nyochaa ọrụ enzymatic nke beta-galactosidase site na nha nke ikuku fluorescence.Hydrolysis nke FMG site na beta-galactosidase na-eduga na ntọhapụ nke fluorescein, na-eme ka mmụba nke mgbaàmà fluorescent nke nwere ike ịdị ọnụ.Enwere ike iji FMG dị ka nyocha mkpụrụ ndụ iji mụọ njikọ njikọ nke lectins (protein na-ejikọta kpọmkwem na carbohydrates) na carbohydrates nwere galactose.Enwere ike ịchọpụta njikọ nke FMG-lectin na ọnụ ọgụgụ dabere na mgbanwe nke ikuku fluorescence. N'ozuzu, FMG bụ ngwá ọrụ dị iche iche na-amụ ọrụ enzyme na njirimara carbohydrate, na-enye usoro dị mma na nke dị nro iji tụọ fluorescence na nyochaa usoro ndu ndị a.


Nkọwa ngwaahịa

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Ngwa na Mmetụta

Fluorescein mono-beta-D-galactopyranoside (FMG) bụ molekul a na-ejikarị na nyocha nke ndu dị ka mkpụrụ iji chọpụta ọnụnọ na ọrụ nke beta-galactosidase enzyme.FMG bụ ihe ewepụtara na lactose shuga ma jikọtara ya na molekul fluorescein.

Mmetụta bụ isi nke FMG bụ na beta-galactosidase na-edozi ya kpọmkwem, enzyme nke na-akụda lactose n'ime galactose na glucose.Nke a enzymatic hydrolysis nke FMG na-eduga na ntọhapụ nke fluorescein, nke na-ebunye mgbama fluorescence siri ike.

Ngwa bụ isi nke FMG bụ n'ịchọpụta na tụọ ọrụ beta-galactosidase n'ụdị dị iche iche.A na-achọta enzyme a n'ọtụtụ ihe ndị dị ndụ, gụnyere nje bacteria na mkpụrụ ndụ mammalian, na ọrụ ya nwere ike igosi usoro dị iche iche nke cell na ụzọ metabolic.

Site n'iji FMG dị ka mkpụrụ, enwere ike tụọ ọrụ beta-galactosidase site na nyochaa fluorescence nke fluorescein a tọhapụrụ na-ewepụta.Enwere ike ịme nha a n'ụdị nhazi nnwale dị iche iche, gụnyere nyocha in vitro na ọmụmụ ihe onyonyo ndụ ndụ.

Ọzọkwa, enwere ike iji FMG dị ka ngwá ọrụ iji mụọ nkesa na nhazi nke beta-galactosidase n'ime sel.Site n'iji usoro microscopy fluorescent, ndị nchọpụta nwere ike iji anya nke uche hụ nhụsianya FMG na-ewepụta n'elu hydrolysis, na-enye ha ohere soro ọrụ oghere na nwa oge nke beta-galactosidase.

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142 (1)

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CAS Mba. 102286-67-9
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