FLUORESCEIN MONO-BETA-D- GALACTOPYRANOSIDE CAS:102286-67-9

Fluorescein mono-beta-D-galactopyranoside, nke a makwaara dị ka FMG, bụ ihe na-eme ka ọ dị ka ihe na-eme ka ọ dị n'ọtụtụ nnwale biochemical na cell bayooloji. A na-esi na methyl-beta-D-galactopyranoside enweta ya site na ijikọ ya na fluorescein molecule. A na-ejikarị FMG amụ ọrụ nke beta-galactosidase, enzyme nke na-eme ka hydrolysis nke lactose ghọọ galactose na glucose. Site na iji FMG dị ka ihe na-eme ka ọ dị n'ime, ndị nchọpụta nwere ike inyocha ọrụ enzymatic nke beta-galactosidase site na nha nke mmepụta fluorescence. Hydrolysis nke FMG site na beta-galactosidase na-eduga na ntọhapụ nke fluorescein, na-ebute mmụba na mgbaàmà fluorescent nke enwere ike ịtụle. A na-ejikwa ihe a eme ihe iji nyochaa nnabata carbohydrate na mmekọrịta. Enwere ike iji FMG dị ka ihe nyocha molecular iji mụọ njikọ nke lectins (protein ndị na-ejikọ kpọmkwem na carbohydrates) na carbohydrates nwere galactose. Enwere ike ịchọpụta ma tụọ njikọ nke FMG-lectin complexes dabere na mgbanwe na mwepụta fluorescence. N'ozuzu, FMG bụ ngwa ọrụ dị iche iche n'ịmụ ọrụ enzyme na njirimara carbohydrate, na-enye ụzọ dị mfe ma dị nro iji tụọ fluorescence na inyocha usoro bayoloji ndị a.

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Nkọwa Ngwaahịa

Akara Ngwaahịa

Ngwa na Mmetụta

Fluorescein mono-beta-D-galactopyranoside (FMG) bụ molekul a na-ejikarị eme nnyocha gbasara ihe ndị dị ndụ dịka ihe e ji achọpụta ọnụnọ na ọrụ nke enzyme beta-galactosidase. FMG bụ ihe sitere na shuga lactose ma jikọta ya na molekul fluorescein.

Isi ihe FMG na-eme bụ na beta-galactosidase, enzyme nke na-agbaji lactose ka ọ bụrụ galactose na glucose, na-eme ka ọ dịghachi ndụ. Enzymatic hydrolysis nke FMG a na-eduga na mwepụta nke fluorescein, nke na-ewepụta akara fluorescence siri ike.

Isi ihe e ji FMG eme bụ ịchọpụta ma tụọ ọrụ beta-galactosidase n'ọtụtụ ihe atụ. Enzyme a dị n'ọtụtụ ihe ndị dị ndụ, gụnyere nje bacteria na mkpụrụ ndụ anụmanụ, ọrụ ya nwekwara ike igosi usoro sel dị iche iche na ụzọ metabolic.

Site n'iji FMG dị ka ihe na-eme ka ihe dị n'ime ya pụta, a pụrụ ịtụ ọrụ beta-galactosidase site n'ileba anya na fluorescence nke fluorescein ewepụtara. Enwere ike iji nha a mee ihe n'ọtụtụ usoro nnwale, gụnyere nyocha in vitro na ọmụmụ ihe onyonyo mkpụrụ ndụ.

Ọzọkwa, enwere ike iji FMG mee ihe dị ka ngwaọrụ iji mụọ nkesa na ebe beta-galactosidase dị n'ime sel. Site na iji usoro microscopy fluorescent, ndị nchọpụta nwere ike ịhụ ìhè fluorescence nke FMG na-ewepụta mgbe hydrolysis gasịrị, na-enye ha ohere ịchọpụta ọrụ oghere na oge nke beta-galactosidase.

Ihe Nlereanya Ngwaahịa

Nkwakọ ngwaahịa:

Ozi Mgbakwunye:

Ihe mejupụtara ya	C26H22O10
Nnwale	99%
Ọdịdị	Ntụ ntụ ọcha
Nọmba CAS	102286-67-9
Nkwakọ ngwaahịa	Obere na nnukwu
Ndụ nchekwa	afọ 2
Nchekwa	Debe n'ebe dị jụụ ma kpọọ nkụ
Asambodo	ISO.