Gly-Pro p-nitroanilide p-toluenesulfonate nnu CAS:65096-46-0

Nnu Gly-Pro p-nitroanilide p-toluenesulfonate bụ ihe mejupụtara kemịkalụ eji eme nnwale enzymatic iji tụọ ọrụ nke peptidases proline. Ọ mejupụtara dipeptide Gly-Pro nke ejikọtara na otu p-nitroanilide, nke enwere ike iwepụta site na ọrụ nke peptidases proline-specific.

N'ime nnwale enzymatic, a na-agbakwunye ihe mejupụtara a na ihe nlele nwere enzyme nke mmasị. Enzyme ahụ na-ekewa njikọ dị n'etiti glycine (Gly) na proline (Pro), na-ewepụta otu p-nitroanilide. Enwere ike ịtụ p-nitroanilide ewepụtara site na iji spectrophotometric, na-enye ohere ịchọpụta ọrụ enzyme ahụ.

Ụdị nnu p-toluenesulfonate nke Gly-Pro p-nitroanilide na-enye nkwụsi ike na mmiri mmiri, na-eme ka ojiji ya dị mfe na nnwale enzymatic..

Nye iwu ma ọ bụ rịọ maka ọnụahịa

Nkọwa Ngwaahịa

Akara Ngwaahịa

Ngwa na Mmetụta

Mmetụta: E mere nnu Gly-Pro p-nitroanilide p-toluenesulfonate kpọmkwem ka ọ bụrụ ihe ndabere maka peptidases nke proline. Enzymes ndị a na-ekewa njikọ peptide dị n'etiti glycine (Gly) na proline (Pro) residues, na-ewepụta p-nitroanilide (pNA) dị ka ngwaahịa. Mkpụrụ ndụ pNA na-amịkọrọ ìhè na ogologo ebili mmiri kpọmkwem, na-enye ohere maka nha nke pNA ewepụtara na atụmatụ nke ọrụ enzyme.

Ojiji: A na-eji nnu Gly-Pro p-nitroanilide p-toluenesulfonate eme ihe n'ọtụtụ ebe nyocha, gụnyere biochemistry, enzymology, na nchọpụta ọgwụ, maka ojiji ndị a:

Nnyocha ọrụ enzyme: A na-ejikarị ya atụ ọrụ nke peptidases proline-specific, gụnyere dipeptidyl peptidases (DPPs) na prolyl endopeptidases (PEPs).

Ihe mgbochi enzyme a na-enyocha: Enwere ike iji nnu Gly-Pro p-nitroanilide p-toluenesulfonate nyochaa ike mgbochi nke ihe ndị mejupụtara ma ọ bụ ọgwụ na ọrụ peptidase nke proline. Mbelata ọ bụla na mwepụta nke pNA na-egosi ọnụnọ nke ihe mgbochi.

Ọmụmụ gbasara kinetics nke enzyme: Site n'ịgbanwe njupụta nke nnu Gly-Pro p-nitroanilide p-toluenesulfonate na ịtụ ọnụego ntọhapụ pNA, enwere ike ịchọpụta paramita kinetics nke enzyme dị ka Km (Michaelis-Menten constant) na Vmax (ọsọ kachasị elu).

Ngwa nchọpụta: Ọrụ nke peptidases nke proline nwere ike ịdị mkpa na nnwale nchọpụta maka ọrịa na ọnọdụ dị iche iche, dịka ọrịa na kansa. Enwere ike iji nnu Gly-Pro p-nitroanilide p-toluenesulfonate dị ka ngwaọrụ iji nyochaa ọkwa ọrụ nke enzymes ndị a na ihe nlele ndị ọrịa.

Nkwakọ ngwaahịa:

Ozi Mgbakwunye:

Ihe mejupụtara ya	C20H24N4O7S
Nnwale	99%
Ọdịdị	Ntụ ntụ ọcha
Nọmba CAS	65096-46-0
Nkwakọ ngwaahịa	Obere na nnukwu
Ndụ nchekwa	afọ 2
Nchekwa	Debe n'ebe dị jụụ ma kpọọ nkụ
Asambodo	ISO.