HEPES CAS: 7365-45-9 Onye nrụpụta ọnụahịa

Nchikota pH: A na-eji HEPES eme ihe n'ọtụtụ ebe dị ka onye na-ebufe ihe iji jikwaa pH kwụsiri ike na mgbasa ozi omenala cell yana nyocha ndu.Ọ na-arụ ọrụ nke ọma na pH physiological nke 6.8 ruo 8.2, na-eme ka ọ dị mma maka idobe ọnọdụ kachasị mma maka usoro ndu.

Omenala cell: A na-agbakwunye HEPES na mgbasa ozi omenala cell iji mee ka ọkwa pH kwụsie ike, na-enye ebe kwesịrị ekwesị maka uto cell na ịnọgide na-enwe ike ndụ cell.Ọ na-enyere aka igbochi mgbanwe pH nke nwere ike imetụta omume cell na nsonaazụ nnwale na-adịghị mma.

Enzyme assays: A na-ejikarị HEPES eme ihe dị ka ihe nchekwa na mmeghachi omume enzymatic n'ihi ikike ya idowe otu pH.Ọ na-enyere aka ịnọgide na-enwe ezigbo ọrụ enzymatic na nkwụsi ike, si otú a na-eme ka izi ezi na ntụkwasị obi nke nyocha enzyme.

Ọmụmụ ihe protein: A na-eji HEPES n'ọrụ na nnwale protein dị iche iche, gụnyere nchacha protein, kristal protein na nyocha nhazi protein.Ọ na-enyere aka mee ka pH kwụsie ike ma nọgide na-enwe mpịakọta protein na nkwụsi ike n'oge nnwale ndị a.

Electrophoresis: HEPES na-achọta ngwa na usoro electrophoresis gel, dị ka SDS-PAGE na agarose gel electrophoresis.A na-eji ya dị ka onye na-ebufe ihe n'ime ihe nchekwa na-agba ọsọ, na-enye ebe pH kwụsiri ike maka nkewa na nyocha nke biomolecules.

Usoro ọgwụ: A na-eji HEPES na-emepụta ọgwụ na ngwaahịa biotechnological.Ọ na-enyere aka ịnọgide na-enwe nkwụsi ike na iguzosi ike n'ezi ihe nke ihe ndị na-arụ ọrụ, na-eme ka arụmọrụ ha dị mma na ndụ nchekwa.