HEPES-Na CAS:75277-39-3 Ọnụahịa Onye Mmepụta

Ihe na-eme ka nchekwa dị mfe: A na-ejikarị nnu sodium HEPES eme ihe dị ka ihe na-eme ka nchekwa dị mfe na nnwale bayọlọji na nke kemịkalụ. Ọ na-enyere aka ịnọgide na-enwe oke pH kwụsiri ike, ọkachasị na oke physiological (pH 7.2-7.6). Ike nchekwa ya na-eme ka ọ baa uru n'ịnọgide na-enwe ọnọdụ kwesịrị ekwesị maka mmeghachi omume enzymatic dị iche iche, ọdịbendị sel, na usoro bayoloji molekul.

Omenala mkpụrụ ndụ: A na-eji nnu sodium HEPES eme ihe nke ukwuu dị ka ihe na-egbochi mkpụrụ ndụ ihe nketa. Ikike ịnọgide na-enwe pH kwụsiri ike dị mkpa maka uto na ndụ nke mkpụrụ ndụ. A na-ahọrọ HEPES karịa ihe ndị ọzọ na-egbochi mkpụrụ ndụ ihe nketa ebe ọ bụ na ọ naghị egosi mgbanwe dị ukwuu na pH mgbe e tinyere ya na CO2 ikuku.

Ọmụmụ Enzyme: Nnu sodium HEPES bara uru nke ukwuu n'ọmụmụ enzymatic ebe achọrọ gburugburu pH na-agbanwe agbanwe ma na-achịkwa. Ọ na-egbochi nnukwu mgbanwe na pH n'oge mmeghachi omume enzymatic, na-eme ka ọrụ enzyme kacha mma.

Electrophoresis: A na-ejikarị nnu sodium HEPES eme ihe dị ka ihe na-egbochi ihe n'ọtụtụ usoro electrophoretic dị iche iche dịka electrophoresis gel polyacrylamide (PAGE) na agarose gel electrophoresis. Ọ na-enyere aka ịnọgide na-enwe pH nke ihe nchekwa ahụ, nke dị oke mkpa maka ikewapụ na inyocha nucleic acids na protein.

Nnyocha Biochemical: A na-ejikarị nnu sodium HEPES eme ihe n'ọtụtụ nyocha biochemical, gụnyere nnwale enzyme, nyocha nha protein, na nyocha spectrophotometric. Ọ na-enyere aka ịnọgide na-enwe oke pH achọrọ maka nsonaazụ ziri ezi na nke a pụrụ imegharị.

Usoro ọgwụ: A na-ejikwa nnu sodium HEPES eme ihe n'ụdị ọgwụ dị ka ihe na-egbochi ọgwụ iji mee ka usoro ọgwụ kwụsie ike ma nọgide na-enwe oke pH achọrọ.