N-Ethylmaleemide CAS:128-53-0 Ọnụahịa Onye Mmepụta

Mgbanwe Protein: NEM na-agbanwe ihe fọdụrụ na cysteine ​​na protein site n'ịmepụta mgbakwunye covalent. Mgbanwe a nwere ike imetụta ọrụ protein, nhazi, na mmekọrịta. Mmeghachi omume NEM na otu sulfhydryl na-eme ka ọ bụrụ ngwaọrụ bara uru maka ịmụ ọrụ protein na usoro dabere na thiol.

Mgbochi nke Enzymes: Ọtụtụ enzymes na-adabere na otu thiol maka ọrụ catalytic ha. Site na imeghachi omume na otu thiol, NEM nwere ike igbochi enzymes ụfọdụ kpọmkwem. A na-eji nke a eme ihe nke ukwuu iji mụọ kinetics nke enzyme, ghọta usoro enzymatic, na ịchọpụta ihe ndị nwere ike igbochi enzyme.

Ọmụmụ Mmekọrịta Protein na Protein: A na-ejikarị NEM eme nnyocha banyere mmekọrịta protein na protein. Site na igbochi ihe fọdụrụ na cysteine ​​na-emegharị ahụ, NEM na-egbochi njikọ disulfide n'etiti protein na-emekọ ihe, si otú a na-emebi mmekọrịta ha. Nke a na-enye ndị nchọpụta ohere ịmụ ọrụ nke cysteine ​​kpọmkwem na mmekọrịta protein na protein.

Ọmụmụ Ihe gbasara Redox Regulation: A na-eji NEM eme ihe n'ịmụ usoro nhazi redox na protein. Site n'ịgbanwe cysteines na-arụ ọrụ, NEM nwere ike ime ka nhazi protein ụfọdụ guzosie ike ma gbochie oxidation ma ọ bụ mbelata nke ihe fọdụrụ na cysteine. Nke a na-enyere aka ịghọta ọrụ nke redox signaling na ụzọ nchịkwa na-emetụta redox.

Ọmụmụ Njikọta: Enwere ike iji NEM mee ihe dị ka ihe na-ejikọ njikọ iji mee ka mgbagwoju protein ma ọ bụ mmekọrịta protein na protein sie ike. Site n'ịgbanwe ihe fọdụrụ na cysteine, NEM nwere ike ịmepụta njikọ covalent n'etiti protein na-akpakọrịta, na-enye ndị nchọpụta ohere inyocha mgbagwoju protein na nhazi ha. Usoro a bara uru karịsịa na bayoloji nhazi na ọmụmụ proteomics.

Nchọpụta Ọgwụ: N'ihi ikike ya nwere iji nhọrọ na ihe fọdụrụ n'ime cysteine ​​emekọrịta ihe, ejirila NEM mee nchọpụta nchọpụta ọgwụ. Enwere ike iji ya chọpụta ma kọwaa ihe ndị nwere ike ịbụ ọgwụ ma ọ bụ mụọ kpọmkwem ọgwụ ndị a ga-eji na-enye protein ndị bara ụba na cysteine.