NBD chloride CAS:10199-89-0

NBD chloride, nke a makwaara dị ka 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole, bụ agba fluorescent nke a na-ejikarị eme ihe na kemịkalụ na ihe ndị dị ndụ. Ọ bụ nke otu nitrobenzoxadiazole (NBD) derivatives, nke nwere ike igosipụta ike fluorescence.

A na-amata NBD chloride site n'ike ya ịmepụta njikọ covalent na nucleophiles, dị ka primary amines, thiols, na hydroxyl groups. Njirimara a na-enye ohere iji ya dị ka ihe nnọchianya akara maka biomolecules, gụnyere protein, peptides, nucleic acid, na obere molecules ndị ọzọ.

Mgbe NBD chloride meghachiri omume na nucleophile, ọ na-enweta mmeghachi omume nnọchi, nke na-eme ka e nwee ngwaahịa kwụsiri ike nke ejiri akara NBD mee. Ngwaahịa a e ji akara mee na-egosipụta nnukwu mwepụta fluorescence, nke na-eme ka ọ baa uru maka usoro nchọpụta na onyonyo dị iche iche.

Enwere ike iji ìhè ultraviolet (UV) mee ka ìhè fluorescence nke molekul ndị e ji akara NBD pụta ìhè, a na-ahụkarịkwa ìhè ndị e wepụtara na ihe dịka 530 nm. Ogologo ebili mmiri a na-adaba n'ime mpaghara akwụkwọ ndụ akwụkwọ ndụ nke ụdị a na-ahụ anya, ebe nnyonye anya nchọpụta sitere na ihe nlele ihe ndị dị ndụ dị obere.

Nye iwu ma ọ bụ rịọ maka ọnụahịa

Nkọwa Ngwaahịa

Akara Ngwaahịa

Ngwa na Mmetụta

Akara Covalent: NBD chloride nwere ike ịmepụta njikọ covalent na otu nucleophilic na biomolecules, dị ka primary amines, thiols, na hydroxyl groups. Nke a na-enye ohere maka akara kpọmkwem nke biomolecules ndị a maka ebumnuche nchọpụta na onyonyo.

Mmụba ọkụ: Mgbe NBD chloride meghachiri omume na nucleophiles, ọ na-emepụta ngwaahịa fluorescent nke e ji akara NBD mee. Ngwaahịa a e ji akara mee na-egosipụta ike fluorescent, nke na-eme ka ọ baa uru maka ịhụ ihe na ịchọpụta ihe ndị e ji akara mee.

Mmetụta na Nkọwapụta: Mwepụ fluorescence nke ngwaahịa ndị e ji akara NBD mee na-enwe mmetụta dị ukwuu, na-enye ohere ịchọpụta obere mkpụrụ ndụ ihe nketa akpọrọ. Ọzọkwa, nhazi njikọ covalent n'etiti NBD chloride na nucleophiles na-enye nkọwapụta, ebe ọ na-eme ka agba ahụ sie ike ma dị iche na biomolecule ahụ a na-achọ.

Akara biomolecule: Enwere ike iji NBD chloride mee akara biomolecule dị iche iche, gụnyere protein, peptides, nucleic acid, na obere molecule ndị ọzọ. Nke a na-enyere ndị nchọpụta aka inyocha ebe ha si bịa, ike ha, na mmekọrịta ha na biomolecules ndị a na sistemụ bayoloji.

Nyocha Fluorescence: Enwere ike iji ihe ngosi fluorescence mee ihe iji hụ ihe ndị dị ndụ e ji akara NBD. Usoro a na-enye ohere ka a hụ ihe ndị dị ndụ e ji akara mee n'ọkwa sel maọbụ nke dị n'okpuru sel, na-enye ozi bara uru gbasara nkesa na omume ha.

Sistemụ mmiri: Enwere ike inyocha ma tụọ ọnụọgụgụ biomolecules ejiri akara NBD mee site na iji cytometry mmiri. Usoro a na-enye ohere maka nyocha dị elu nke mkpụrụ ndụ ma ọ bụ ihe ndị e ji akara mee, na-enye ozi gbasara ọkwa ngosipụta ma ọ bụ ihe ndị dị n'elu nke biomolecules a kpọrọ aha.

Akara na nsochi protein: Enwere ike iji NBD chloride depụta ihe fọdụrụ amino acid ma ọ bụ otu ọrụ dị na protein. Nke a na-enyere ndị nchọpụta aka ịmụ banyere nhazi protein, mpịachi, ike, na mmekọrịta n'oge.

Nkwakọ ngwaahịa:

Ozi Mgbakwunye:

Ihe mejupụtara ya	C6H2ClN3O3
Nnwale	99%
Ọdịdị	Ntụ ntụ odo
Nọmba CAS	10199-89-0
Nkwakọ ngwaahịa	Obere na nnukwu
Ndụ nchekwa	afọ 2
Nchekwa	Debe n'ebe dị jụụ ma kpọọ nkụ
Asambodo	ISO.