Dithiothreitol (DTT) bụ ihe a na-ejikarị ebelata ihe, nke a makwaara dị ka mgbakwunye akwụkwọ ndụ akwụkwọ ndụ ọhụrụ.Ọ bụ obere ngwakọta organic molekụla nwere otu mercaptan abụọ (-SH).N'ihi mbelata ihe onwunwe na nkwụsi ike ya, a na-eji DTT eme ihe na biochemistry na nyocha ihe ọmụmụ ihe omimi.
Isi ọrụ nke DTT bụ ibelata njikọ disulfide na protein na biomolecules ndị ọzọ.Njikọ disulfide bụ akụkụ dị mkpa nke mpịakọta protein na nkwụsi ike, mana n'okpuru ọnọdụ nnwale ụfọdụ, dị ka nyocha SDS-PAGE reducible, protein recombination na mpịachi, ọ dị mkpa iji belata njikọ disulfide na otu thiol abụọ iji kpughee usoro oghere nke oghere. protein ahụ.DTT nwere ike meghachi omume na njikọ disulfide iji belata ha na ndị otu mercaptan, si otú a na-emepe oghere oghere nke protein ahụ ma mee ka ọ dị mfe nyocha na nhazi.
A pụkwara iji DTT chebe ọrụ enzyme na nkwụsi ike.N'ime mmeghachi omume ụfọdụ nke enzyme-catalyzed, ọrụ nke enzyme nwere ike belata site na oxidant.DTT nwere ike meghachi omume na oxidants iji belata ha na ihe ndị na-adịghị emerụ ahụ, si otú a na-echebe ọrụ na nkwụsi ike nke enzyme.
Tụnyere ihe ndị na-ebelata mbelata ọdịnala dị ka β-mercaptoethanol (β-ME), a na-ahụta DTT dị ka onye na-ebelata ihe nchekwa ma kwụsie ike karị.Ọ bụghị naanị na ọ kwụsiri ike na ngwọta mmiri, kamakwa ọ na-ejigide ihe ndị na-ebelata ya n'okpuru ọnọdụ okpomọkụ dị elu na ọnọdụ acid-base.
Ojiji nke DTT dịtụ mfe.N'ozuzu, DTT na-agbaze na nchekwa kwesịrị ekwesị wee tinye ya na usoro nnwale.Ekwesịrị ikpebi ntinye kachasị mma nke DTT dị ka nnwale a kapịrị ọnụ, a na-ejikarị ya na oke 0.1-1mM.Nleta dị ala nwere ike ibelata mmetụta ọjọọ na uto cell ma nwee ike ibelata cytotoxicity n'ihi oke iwepụta protein ndị e lekwasịrị anya.Mmụba dị elu nwere ike ime ka ibu metabolism dị elu nke cell, na-emetụta uto cell na nrụpụta okwu.
Uzo esi ekpebi itinye uche kacha mma nwere ike ịbụ inyocha ọkwa ngosipụta nke protein ebumnuche site na ịme ule nnabata IPTG na mkpokọta dị iche iche.Enwere ike ime ule omenaala pere mpe site na iji ọtụtụ ọnụọgụ IPTG (dịka 0.1 mM, 0.5 mM, 1 mM, wdg) enwere ike nyochaa mmetụta okwu na ntinye dị iche iche site na ịchọpụta ọkwa ngosipụta nke protein ebumnuche (dịka Western Western). ntụpọ ma ọ bụ fluorescence nchọpụta).Dabere na nsonaazụ nnwale ahụ, a na-ahọrọ itinye uche na mmetụta ngosipụta kachasị mma dị ka ntinye kachasị mma.
Na mgbakwunye, ị nwekwara ike na-ezo aka na akwụkwọ ndị dị mkpa ma ọ bụ ahụmahụ nke ụlọ nyocha ndị ọzọ iji ghọta ụdị ịta ahụhụ IPTG a na-ejikarị eme ihe n'okpuru ọnọdụ nnwale ndị yiri ya, wee kwalite ma gbanwee dịka mkpa nyocha.
Ọ dị mkpa iburu n'obi na ntinye kachasị mma nwere ike ịdịgasị iche dabere na usoro okwu dị iche iche, protein ndị e lekwasịrị anya, na ọnọdụ nnwale, n'ihi ya, ọ kacha mma ịkwalite n'otu n'otu.
Na nchịkọta, DTT bụ onye na-ebelata ihe a na-ejikarị eme ihe nke enwere ike iji belata njikọ disulfide na protein na biomolecules ndị ọzọ yana iji chebe ọrụ enzyme na nkwụsi ike.A na-eji ya eme ihe n'ọtụtụ ebe na nyocha biochemistry na ihe ndị dị ndụ.
Oge nzipu: Sep-28-2023