E nwere ọtụtụ ndị dike a na-akpọghị aha gburugburu anyị, ndị yiri ka ha bụ ndị nkịtị, mana n'eziokwu ha na-enye anyị nnukwu aka n'olu dị ala. Proteinase K bụ "dike a na-akpọghị aha" n'ụlọ ọrụ nchọpụta molekul, ọ bụ ezie na e jiri ya tụnyere "onye ukwu na onye dị ike" n'ụlọ ọrụ ahụ, proteinase K dị obere nke na anyị elegharala mkpa ya anya ogologo oge. Ka ọrịa ọhụrụ nke okpueze malitere, ọchịchọ maka proteinase K amụbaala, ihe ndị dị n'ime obodo na mba ofesi dị nnọọ n'azụ oriri, onye ọ bụla ghọtara na proteinase K dị oke mkpa.
Kedu ihe bụ ojiji nke proteinase K?
Proteinase K bụ serine protease nwere ọrụ enzyme proteolytic ma nwee ike ịnọgide na-arụ ọrụ n'ọtụtụ ebe (pH (4-12.5), nnukwu nnu, okpomọkụ dị elu nke 70°C, wdg). Na mgbakwunye, SDS, urea, EDTA, guanidine hydrochloride, guanidine isothiocyanate, wdg anaghị egbochi ọrụ nke proteinase K, yana obere ihe nhicha ahụ nwekwara ike ime ka ọrụ nke proteinase K ka mma. N'ọgwụgwọ ahụike (nje virus na nje bacteria), nri (nsị anụ), akpụkpọ anụ (nsị ntutu isi), ime mmanya (nkọwa mmanya), nkwadebe amino acid (nku mebiri emebi), mwepụta nucleic acid, in situ hybridization, wdg, proteinase K E nwere ngwa. Ngwa a na-ejikarị eme ihe bụ mwepụta nucleic acid.
Proteinase K nwere ike itinye enzyme ụdị protein niile dị na ihe nlele ahụ, gụnyere histones ndị ahụ ejikọtara na nucleic acid, ka e wee nwee ike ịtọhapụ nucleic acid site na ihe nlele ahụ ma tọhapụ ya n'ime ihe ewepụtara, na-eme ka usoro ọzọ nke mwepụ na nhicha dị mfe. N'ịchọpụta nucleic acid nke nje virus, proteinase K bụ otu n'ime ihe dị mkpa na ngwọta nnwale nje ahụ. Proteinase K nwere ike ịgbawa ma gbanyụọ protein mkpuchi nke nje ahụ, nke dị nchebe n'oge njem na nchọpụta; na mgbakwunye, proteinase K nwekwara ike imebi RNase na-egbochi mmebi nke RNA nje ma na-eme ka nchọpụta nucleic acid dị mfe.
Ama ama nke proteinase K n'otu abalị
Ma n'ihe gbasara nnyocha sayensị ma ọ bụ n'ihe gbasara IVD, mwepụta nucleic acid bụ nnwale kachasị mkpa, yabụ proteinase K abụrụla ihe dị oke mkpa mgbe niile. Agbanyeghị, n'oge gara aga, a machaghị proteinase K nke ọma karịa ọrụ ya. Akụkụ dị ukwuu nke nke a bụ n'ihi na mmekọrịta ọkọnọ na ọchịchọ nke proteinase K siri ike nke ukwuu. Ọ bụ mmadụ ole na ole ga-eche na inye proteinase K ga-abụ nsogbu.
Site na ntiwapụ nke ọrịa okpueze ọhụrụ a, ọchịchọ maka nnwale nucleic acid amụbaala. Ka ọ na-erule ngwụcha June 2020, China emechaala ihe fọrọ nke nta ka ọ bụrụ nde 90 nke ule okpueze ọhụrụ, ọnụọgụ a dịkwa egwu karịa n'ụwa niile. Na nnwale mwepụta nucleic acid, ntinye ọrụ nke proteinase K dị ihe dị ka 50-200 μg/mL. N'ozuzu, ọ na-ewe ihe dị ka 100 μg nke proteinase K iji wepụta otu ihe atụ nke nucleic acid. N'oge ojiji, iji mee ka arụmọrụ nke mwepụta nucleic acid dịkwuo elu, a na-ejikarị Proteinase K eme ihe n'ọtụtụ buru ibu. Nchọpụta nucleic acid nke coronavirus ọhụrụ ewetala nnukwu ọchịchọ proteinase K. Nguzozi mbụ nke proteinase K mebiri ngwa ngwa, proteinase K ghọkwara ihe dị mkpa maka mgbochi ọrịa n'otu ntabi anya.
Ihe isi ike dị na mmepụta nke proteinase K
Ọ bụ ezie na n'oge ọrịa a na-efe efe, ndị mmadụ ewerela uru dị mkpa nke proteinase K, ọ bụ ihe ihere na n'ihi oke obere proteinase K, ụlọ ọrụ ole na ole dị n'ime obodo etinyela aka na mmepụta proteinase K. Mgbe ndị mmadụ chọrọ ịmepụta proteinase K N'oge usoro mmepụta ahụ, achọpụtara na proteinase K bụ protein pụrụ iche. Ọ na-esiri ike nke ukwuu ịgbasa ikike mmepụta nke proteinase K n'ime obere oge.
Mmepụta proteinase K buru ibu na-eche nsogbu ndị a ihu
1. Ngosipụta dị ala
Proteinase K nwere ike imebi ọtụtụ protein n'ụzọ na-adịghị mkpa ma kpatara nnukwu nsí na mkpụrụ ndụ nnabata ahụ. Ya mere, ọkwa ngosipụta nke proteinase K na-adịkarị ala nke ukwuu. Nnyocha nke sistemụ ngosipụta na ụdị ndị na-egosipụta proteinase K nke ọma na-achọkarị usoro ogologo oge.
2. Ihe fọdụrụ nke agba na asịd nucleic
Nnukwu ịgbaze ihe na-eme ka ọtụtụ agba na ihe fọdụrụ n'ime nucleic acid dị na ya pụta. Ọ na-esiri ike iwepụ ihe ndị a na-adịghị mma site na iji usoro nhicha dị mfe, nhicha dị mgbagwoju anya na-emekwa ka ọnụ ahịa dịkwuo elu ma na-ebelata ọnụego mgbake.
3. Enweghị ntụkwasị obi
Proteinase K adịghị ike nke ọma, ọ nwere ike itinye enzyme n'onwe ya, ọ na-esikwa ike ịchekwa ya nke ọma na okpomọkụ 37°C ruo ogologo oge na-enweghị ihe nchebe.
4. Ọ dị mfe ịgbapụta
Mgbe ị na-akwadebe ntụ ntụ proteinase K nke a kpọnwụrụ akpọnwụ, iji hụ na ọdịnaya siri ike nke proteinase K dị na ntụ ntụ a kpọnwụrụ akpọnwụ buru ibu, ọ dị mkpa ịgbakwunye ihe nchebe a kpọnwụrụ akpọnwụ na oke njupụta, mana mgbe njupụta nke proteinase K ruru 20mg/mL na karịa, ọ dị mfe. Nchikota ahụ na-emepụta ihe na-akpali akpali, nke na-eweta nnukwu nsogbu na ịkpọnwụ nke proteinase K nke nwere nnukwu njupụta siri ike.
5. Nnukwu itinye ego
Proteinase K nwere ọrụ protease siri ike ma nwee ike ime ka protease ndị ọzọ dị na mmiri mmiri n'ime ụlọ nyocha. Ya mere, proteinase K chọrọ ebe mmepụta pụrụ iche, akụrụngwa, na ndị ọrụ maka nyocha na mmepe na mmepụta.
Ngwakọta proteinase K nke XD BIOCHEM
XD BIOCHEM nwere ikpo okwu ngosipụta na nhicha protein tozuru oke, ọ nwekwara ahụmịhe bara ụba n'igosipụta na ịsacha protein ndị a na-akpọ recombinant na imeziwanye usoro mmepụta. Site na nhazi ngwa ngwa nke ndị otu nyocha na mmepe, emeriela usoro mmepụta buru ibu nke proteinase K. Mmepụta kwa ọnwa nke ntụ ntụ akpọnwụ akpọnwụ karịrị 30 KG. Ngwaahịa a nwere arụmọrụ kwụsiri ike, ọrụ enzyme dị elu, na enweghị ihe fọdụrụ na cytochrome na nucleic acid. Nnọọ na kọntaktị XD BIOCHEM Nweta ngwugwu nnwale (E-mail:sales@xdbiochem.comTel: +86 513 81163739).
Ngwọta teknụzụ nke XD BIOCHEM gụnyere
Site na iji njikọta plasmid dị iche iche, a na-ahọrọ ụdị ngosipụta dị elu nwere ọkwa ngosipụta nke 8g/L, nke na-emeri nsogbu nke ọkwa ngosipụta dị ala nke proteinase K.
Site na ntọala nke usoro nhicha ọtụtụ nzọụkwụ, ewepụrụ ihe fọdụrụ na cytochrome na nucleic acid nke proteinase K nke ọma n'okpuru uru ọkọlọtọ.
Site na nyocha dị elu nke usoro nchekwa nchekwa, ahọpụtara ihe nchekwa nke nwere ike ịchekwa proteinase K nke ọma na 37°C.
Ihe nchekwa nyocha na-emeri nsogbu nke na proteinase K dị mfe ịchịkọta ma mee ka ọ dị mmiri mmiri mgbe ọ dị oke, ma na-atọ ntọala maka ihicha oke proteinase K nke nwere ike ime ka ọ kpọọ nkụ.
Ihe nlele XD BIOCHEM proteinase K
Nnwale nkwụsi ike nke XD BIOCHEM proteinase K: agaghị enwe mgbanwe dị ukwuu na ọrụ mgbe ụbọchị 80 gachara n'ọnọdụ okpomọkụ ụlọ
Nnwale nkwụsi ike nke XD BIOCHEM proteinase K: agaghị enwe nnukwu mgbanwe na ọrụ mgbe ụbọchị 80 gachara n'ọnọdụ okpomọkụ ụlọ.
Ntụnyere mmetụta mwepụta nucleic acid nke XD BIOCHEM proteinase K na ngwaahịa ndị na-asọmpi. N'usoro mwepụta nucleic acid, a na-eji XD BIOCHEM na proteinase K asọmpi n'otu n'otu. A na-eji arụmọrụ mwepụta nke proteinase K nke XD BIOCHEM dị elu, uru Ct nke mkpụrụ ndụ ihe nketa a na-elekwasị anya dị ala.
Oge ozi: Disemba-31-2021