P-NITROPHENYL BETA-D-LACTOPYRANOSIDE CAS:4419-94-7

Nchọpụta ọrụ beta-galactosidase: A na-ejikarị PNPG eme nyocha iji tụọ ọrụ beta-galactosidase, enzyme nke na-eme ka hydrolysis nke lactose banye glucose na galactose.Hydrolysis nke PNPG site na beta-galactosidase na-ewepụta molekul p-nitrophenol (pNP), nke enwere ike ịchọpụta spectrophotometrically n'ihi agba odo ya.

Nyocha maka ndị na-egbochi enzyme na ndị na-eme ihe: PNPG nwere ike iji mee ihe na nyocha dị elu iji chọpụta ogige ndị na-emezi ọrụ beta-galactosidase.Site n'ịleta ọnụego nke PNPG hydrolysis na ọnụnọ nke ogige ule dị iche iche, ndị na-eme nchọpụta nwere ike ịchọpụta ihe mgbochi na-ebelata ọrụ enzyme ma ọ bụ ndị na-eme ihe na-eme ka ọrụ enzyme dịkwuo elu.

Ọmụmụ nke kinetics enzyme: Hydrolysis nke PNPG site na beta-galactosidase na-esote Michaelis-Menten kinetics, na-enye ndị na-eme nchọpụta aka ikpebi ihe dị mkpa enzyme paramita dị ka kacha mmeghachi omume ọsọ (Vmax) na Michaelis mgbe niile (Km).Ozi a na-enyere aka n'ịghọta njikọ mkpụrụ nke enzyme na arụmọrụ catalytic.

Ngwa bayoloji ihe omimi: Beta-galactosidase, nke na-ekewa PNPG, bụ nke a na-ejikarị dị ka mkpụrụ ndụ akụkọ ihe mere eme na bayoloji molecular.A na-ejikarị mkpụrụ osisi PNPG chọpụta na iji anya nke uche hụ okwu nke mkpụrụ ndụ onye nta akụkọ, na-enye ụzọ dị mfe ma dị nro iji nyochaa mkpụrụ ndụ ihe nketa na usoro nnwale dị iche iche.