P-NITROPHENYL BETA-D-LACTOPYRANOSIDE CAS:4419-94-7

P-Nitrophenyl beta-D-lactopyranoside, nke a makwaara dị ka PNPG, bụ ihe mejupụtara nke a na-ejikarị eme ihe na nnwale enzymatic iji tụọ ọrụ nke beta-galactosidase, enzyme nke metụtara metabolism carbohydrate. PNPG bụ ihe mejupụtara sịntetik nke enwere ike kewaa site na beta-galactosidase, na-ebute mwepụta nke ngwaahịa agba odo. Enwere ike ịtụle oke hydrolysis nke substrate site na iji spectrophotometric tụọ nnabata nke ngwaahịa ahụ na ogologo ebili mmiri kpọmkwem. Nke a na-enye ndị nchọpụta ohere inyocha ọrụ na kinetics nke beta-galactosidase n'ọtụtụ ọnọdụ, dị ka ịmụ ọrụ enzyme, inyocha maka ndị na-egbochi enzyme ma ọ bụ ndị na-eme ka ihe rụọ ọrụ, ma ọ bụ ịtụle mmetụta nke mgbanwe na ọrụ enzyme.

Nye iwu ma ọ bụ rịọ maka ọnụahịa

Nkọwa Ngwaahịa

Akara Ngwaahịa

Ngwa na Mmetụta

Nchọpụta nke ọrụ beta-galactosidase: A na-ejikarị PNGG eme ihe n'ule iji tụọ ọrụ nke beta-galactosidase, enzyme nke na-eme ka hydrolysis nke lactose ghọọ glucose na galactose. Hydrolysis nke PNGPG site na beta-galactosidase na-ewepụta molekul p-nitrophenol (pNP), nke enwere ike ịchọpụta site na spectrophotometric n'ihi agba odo ya.

Nnyocha maka ihe mgbochi enzyme na ihe na-eme ka ihe rụọ ọrụ: Enwere ike iji PNPG mee ihe na nyocha dị elu iji chọpụta ihe ndị na-agbanwe ọrụ beta-galactosidase. Site n'ịtụle ọnụego nke PNGPG hydrolysis n'ihu ihe dị iche iche nke nnwale, ndị nchọpụta nwere ike ịchọpụta ihe mgbochi ndị na-ebelata ọrụ enzyme ma ọ bụ ihe na-eme ka ọrụ enzyme dịkwuo mma.

Ọmụmụ gbasara kinetics nke enzyme: Mmepụta hydrolysis nke PNPG site na beta-galactosidase na-esochi kinetics nke Michaelis-Menten, na-enye ndị nchọpụta ohere ịchọpụta paramita enzyme dị mkpa dịka ọsọ mmeghachi omume kachasị elu (Vmax) na mgbanwe Michaelis (Km). Ozi a na-enyere aka ịghọta njikọ dị n'etiti enzyme na arụmọrụ catalytic.

Ngwa gbasara bayoloji molekụla: A na-ejikarị Beta-galactosidase, nke na-ekewa PNPG, eme ihe dị ka mkpụrụ ndụ ihe nketa na bayoloji molekụla. A na-ejikarị ihe ndabere PNPG achọpụta ma hụ ngosipụta nke mkpụrụ ndụ ihe nketa onye na-akọ akụkọ, na-enye ụzọ dị mfe ma dị nro iji nyochaa ngosipụta mkpụrụ ndụ ihe nketa na sistemụ nnwale dị iche iche.

Ihe Nlereanya Ngwaahịa

Nkwakọ ngwaahịa:

Ozi Mgbakwunye:

Ihe mejupụtara ya	C18H25NO13
Nnwale	99%
Ọdịdị	Ntụ ntụ ọcha
Nọmba CAS	4419-94-7
Nkwakọ ngwaahịa	Obere na nnukwu
Ndụ nchekwa	afọ 2
Nchekwa	Debe n'ebe dị jụụ ma kpọọ nkụ
Asambodo	ISO.