Phenylgalactoside CAS:2818-58-8

Mmetụta na ọrụ enzyme: A na-ejikarị Phenylgalactoside atụ ọrụ nke enzyme β-galactosidase. Mgbe β-galactosidase na-eme ka phenylgalactoside dị na mmiri, ọ na-ewepụta p-nitrophenol. Enwere ike ịtụ mkpokọta p-nitrophenol n'ọtụtụ, na-enye nghọta banyere ọrụ β-galactosidase. A na-eji mmetụta a eme ihe na ngwa dịka nnwale enzyme na sistemụ nyocha.

Nyocha nke mkpụrụ ndụ ihe nketa: A na-ejikarị Phenylgalactoside eme ihe dị ka ihe ndabere na nnwale bayọlọjik molekul iji mụọ ngosipụta mkpụrụ ndụ ihe nketa. Mkpụrụ ndụ ihe nketa lacZ, nke na-akọwapụta β-galactosidase, na-ejikọta ya na usoro iwu nke mkpụrụ ndụ ihe nketa ndị ọzọ nwere mmasị. Ngosipụta nke mkpụrụ ndụ ihe nketa lacZ na hydrolysis nke phenylgalactoside site na β-galactosidase nwere ike igosi ụdị ngosipụta na ọkwa nke mkpụrụ ndụ ihe nketa a na-amụ.

Sistemụ nyocha: Phenylgalactoside bụ akụkụ dị mkpa nke sistemụ nyocha nke na-eji ọrụ β-galactosidase. Otu ihe atụ a maara nke ọma bụ usoro nyocha acha anụnụ anụnụ-ọcha, nke a na-eji achọpụta mkpụrụ ndụ recombinant ma ọ bụ gbanwere na nnwale bayoloji molekul. Colonies ndị natara DNA recombinant nke ọma ma ọ bụ ndị natara njikọta mkpụrụ ndụ ihe nketa ga-egosipụta β-galactosidase, na-eduga na hydrolysis nke phenylgalactoside na ịmepụta agba acha anụnụ anụnụ.

Nhicha protein: N'ọnọdụ ụfọdụ, enwere ike iji phenylgalactoside dị ka ligand maka njikọta chromatography iji sachaa protein ndị na-ejikọ kpọmkwem na ma ọ bụ ndị β-galactosidase na-arụ ọrụ. Protein nke mmasị nwere ike inwe akara njikọta ma ọ bụ akara njikọta nke nwere ngalaba njikọta β-galactosidase. Site n'ịfefe ngwakọta protein ahụ site na kọlụm nwere phenylgalactoside na-adịghị arụ ọrụ, enwere ike idobe protein achọrọ ma mechaa wepụ ya.