piperazine-1,4-bis (2-ethanesulfonic acid) nnu disodium CAS: 76836-02-7

Mmetụta:

Ngwongwo nchikota: Enwere ike iji PIPES na-edobe ọkwa pH mgbe nile n'ime otu oke, n'ihi na ọ na-arụ ọrụ n'ịkwadebe na pH physiological nke 6.1-7.5.Nke a na-eme ka ọ baa uru na nyocha dị iche iche nke ndu ebe njikwa pH dị oke mkpa.

Nkwụsi ike: PIPES kwụsiri ike karịa oke okpomọkụ, na-eme ka ọ dabara maka ojiji na nnwale emere n'okpuru ọnọdụ dị iche iche.

Ngwa:

Omenala cell: Enwere ike iji PIPES dị ka ihe nchekwa na usoro omenala cell, dị ka idowe pH nke mgbasa ozi ma ọ bụ ihe nchekwa ejiri maka uto na mmezi cell.

Ihe ọmụmụ protein na enzyme: A na-ejikarị PIPES eme ihe na protein na enzyme ọmụmụ iji nọgide na-enwe pH kwụsiri ike n'oge mmeghachi omume dị iche iche, karịsịa ndị metụtara enzymes ma ọ bụ protein nwere mmetụta nke mgbanwe pH nwere ike imetụta.

Electrophoresis: Enwere ike iji PIPES dị ka ihe nchekwa na ngwa electrophoresis gel, na-enyere aka ịnọgide na-enwe ọnọdụ pH kachasị mma maka DNA ma ọ bụ nkewa protein.

Usoro ihe omumu ihe omimi: Enwere ike iji PIPES mee ihe dika ihe nkpuchi n'ime usoro ihe omumu ihe omimi di iche iche, tinyere DNA/RNA mmịpụta, PCR, na usoro DNA, na-eme ka nsonaazụ ziri ezi site na idowe ọnọdụ pH kwụsiri ike.