SYBR GREEN I Nucleic Acid Gel Agba CAS:163795-75-3

Nlekota anya nke DNA gel: A na-etinye ntụpọ SYBR Green I na jel DNA mgbe elekotachara anya, mgbe e kpugheere ya na ìhè UV, ọ na-ejikọ na eriri DNA, na-emepụta fluorescence na-egbuke egbuke. Usoro agba a na-enyere aka ịhụ ma nyochaa iberibe DNA nke e kewara site na nha.

PCR ọnụọgụgụ (qPCR): A na-ejikarị SYBR Green I eme ihe na qPCR iji nyochaa mmụba nke usoro DNA ma ọ bụ RNA n'oge dị adị. Agba ahụ na-ejikọta na DNA nwere eriri abụọ nke e mepụtara n'oge mmụba PCR, a na-atụkwa mmụba nke ike fluorescence na okirikiri mmụba ọ bụla, na-enye ohere maka nhazi nke template nucleic acid mmalite.

Electrophoresis nke jel RNA: Enwere ike iji SYBR Green I mee electrophoresis nke jel RNA, nke na-enye ohere maka ịhụ ihe na nyocha nke iberibe RNA. Ọ bara uru maka nyocha iguzosi ike n'ezi ihe nke RNA yana maka ịmụ ọkwa ngosipụta RNA.

Ịdepụta na ịchọpụta ihe gbasara Nucleic acid: Enwere ike iji SYBR Green I depụta ihe gbasara Nucleic acid site na iji fluorescence. Enwere ike ime nke a site na itinye agba n'oge mmepụta nucleic acid ma ọ bụ site na iji usoro mgbe e mechara akara. Enwere ike iji sistemụ onyonyo fluorescent mee ihe ma chọpụta nucleic acid ndị a kpọrọ aha.