TRIS-Acetate CAS:6850-28-8 Ọnụahịa Onye Mmepụta

TRIS-Acetate, bụ ihe nchekwa a na-ejikarị eme ihe na nnwale bayoloji na biochemical. Ọ bụ ngwakọta nke Tris base na acetic acid, na-ebute ngwọta pH-stable nke a na-eji achịkwa ma na-edobe oke pH achọrọ maka ọtụtụ ojiji. TRIS-Acetate bara uru karịsịa na ọmụmụ DNA na RNA, ebe ọ na-enye gburugburu ebe kwesịrị ekwesị maka ọrụ enzyme, electrophoresis, na gel electrophoresis. Ọ na-enyere aka ịnọgide na-enwe nkwụsi ike na iguzosi ike n'ezi ihe nke nucleic acid n'oge usoro dị iche iche, dị ka usoro DNA, polymerase chain reaction (PCR), na agarose gel electrophoresis. Na mgbakwunye na nyocha nucleic acid, a na-ejikwa TRIS-Acetate na usoro mwepụta na nhicha protein, mwepụta protein membrane, na nnwale ọdịbendị sel. Ike nchekwa ya dị iche iche na-eme ka ọ bụrụ ngwaọrụ bara uru n'ọtụtụ ebe nke nyocha sayensị, na-ahụ na ọnọdụ kacha mma maka mmeghachi omume bayoloji na ịnọgide na-enwe nkwụsi ike nke enzymes na protein.

Nye iwu ma ọ bụ rịọ maka ọnụahịa

Nkọwa Ngwaahịa

Akara Ngwaahịa

Ngwa na Mmetụta

Tris-acetate (TRIS-Acetate) bụ ihe nchekwa a na-ejikarị eme ihe na nnwale bayọlọji na nke biochemical. Ọ nwere ngwakọta nke tris(hydroxymethyl)aminomethane (Tris) na acetic acid, nke na-arụ ọrụ dị ka ihe na-achịkwa pH na ihe na-eme ka ọ kwụsie ike. pH nke ihe nchekwa TRIS-Acetate na-adịkarị site na 7.4 ruo 8.4.
Isi ihe TRIS-Acetate na-eme bụ ịnọgide na-enwe pH kwụsiri ike, nke dị oke mkpa maka ọtụtụ mmeghachi omume bayọlọji na biochemical. Ọ na-arụ ọrụ dị ka ihe nchekwa site n'ibelata mgbanwe ọ bụla dị mkpa na pH nke nwere ike ime n'ihi asịd ma ọ bụ ntọala agbakwunyere n'oge usoro nnwale.
TRIS-Acetate chọtara ọtụtụ ojiji na bayoloji molekul, biochemistry, na bayotechnology:
DNA na RNA Electrophoresis: A na-ejikarị TRIS-Acetate eme ihe dị ka ihe nchekwa na-agba ọsọ na agarose na polyacrylamide gel electrophoresis. Ọ na-enye gburugburu pH kwụsiri ike n'oge nkewa nke iberibe DNA na RNA dabere na nha ha.
Nnyocha Protein: A na-eji ihe nchekwa TRIS-Acetate eme ihe maka electrophoresis protein, dịka SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis). Ọ na-eme ka protein kwụsie ike ma kewaa n'oge usoro ahụ.
Mmeghachi omume Enzyme: A na-ejikarị TRIS-Acetate buffers eme ihe na nyocha na ọmụmụ ihe gbasara enzyme. Ọ na-enye oke pH kachasị mma maka mmeghachi omume enzymatic dị iche iche ma na-enyere aka ịnọgide na-arụ ọrụ enzyme.
Omenala Mkpụrụ na Anụ Ahụ: A na-eji ihe nchekwa TRIS-Acetate eme ihe na mgbasa ozi ọdịbendị mkpụrụ ndụ iji nọgide na-enwe pH kwesịrị ekwesị maka uto na mmụba nke mkpụrụ ndụ. Ọ na-enyere aka ịnọgide na-enwe ọnọdụ ahụike dị mkpa maka ndụ mkpụrụ ndụ.

Nkwakọ ngwaahịa:

Ozi Mgbakwunye:

Ihe mejupụtara ya	C6H15NO5
Nnwale	99%
Ọdịdị	Ntụ ntụ ọcha
Nọmba CAS	6850-28-8
Nkwakọ ngwaahịa	Obere na nnukwu
Ndụ nchekwa	afọ 2
Nchekwa	Debe n'ebe dị jụụ ma kpọọ nkụ
Asambodo	ISO.