Tris(hydroxymethyl)nitromethane CAS:126-11-4

Mmetụta:

Ike Nchekwa: Tris na-arụ ọrụ dị ka ihe nchekwa dị irè n'ihi ikike ya ịnakwere ma ọ bụ inye protons, na-edobe oke pH kwụsiri ike na ngwọta. A na-ejikarị ya eme ihe dị ka ihe bụ isi na sistemụ nchekwa iji mee ka pH nke ihe nlele na mmeghachi omume dịgide.

Ngwa:

Bayọlọjik Molekụla: A na-eji Tris eme ihe nke ukwuu dị ka ihe na-egbochi ihe n'ọtụtụ usoro bayọlọjik molekụla, gụnyere ichebe DNA na RNA, PCR, gel electrophoresis, na nhicha protein. Ọ na-enyere aka ịnọgide na-enwe gburugburu pH kwụsiri ike, na-enye ohere maka ọnọdụ kacha mma maka mmeghachi omume enzymatic na mmekọrịta molekụla.

Omenala Mkpụrụ Ndụ: A na-ejikarị Tris eme ihe na mgbasa ozi osmotic cell iji nọgide na-enwe pH na nguzozi osmotic mgbe niile, na-akwado uto na ndụ mkpụrụ ndụ dị mma.

Kemistri Protein: A na-eji Tris eme ihe na nnwale kemistri protein, dịka ịgbaze protein, nnwale nkwụsi ike protein, na ọmụmụ ihe gbasara njikọ protein-ligand. Ọ na-enyere aka ịnọgide na-enwe oke pH achọrọ, na-ahụ na protein na-apịachi ma na-arụ ọrụ nke ọma.

Enzymology: A na-eji Tris eme ihe n'ọtụtụ nyocha enzymatic iji mee ka ọnọdụ pH dị mkpa maka ọrụ enzymatic dịkwuo mma. Ọ na-eme ka nsonaazụ a pụrụ ịtụkwasị obi na nke a pụrụ ịmụgharị pụta ìhè, na-eme ka a mata nke ọma nke kinetics enzyme na ọmụmụ ihe mgbochi.

Nnyocha Biochemical: A na-eji Tris eme ihe dị ka ihe dị mkpa n'ọtụtụ nyocha biochemical n'ihi ihe ndị na-egbochi ya. Ọ na-ejigide pH na-adịgide adịgide n'oge nyocha colorimetric, spectrophotometric, na enzymatic, na-eme ka izi ezi na ntụkwasị obi nke nsonaazụ ahụ dịkwuo mma.