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X-GAL CAS: 7240-90-6 Onye nrụpụta ọnụahịa

5-Bromo-4-chloro-3-indolyl-β-D-galactoside (X-Gal) bụ ihe a na-ahụkarị chromogenic nke a na-eji na bayoloji molecular na ngwa biochemistry.A na-eji ya eme ihe n'ọtụtụ ebe maka nchọpụta nke mkpụrụ ndụ lacZ, nke na-eme ka enzyme β-galactosidase.


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Ngwa na Mmetụta

Mgbanwe agba: X-Gal na-enwekarị ụcha mana, na hydrolysis site na β-galactosidase, ọ na-atụgharị na-acha anụnụ anụnụ.Mgbanwe agba a na-enye ohere maka nchọpụta anya na ọnụọgụ nke ọrụ β-galactosidase.

Nchọpụta Gene LacZ: A na-eji X-Gal chọpụta mkpụrụ ndụ ma ọ bụ ihe mkpụrụ ndụ ihe nketa na-egosipụta mkpụrụ ndụ lacZ.A na-ejikarị LacZ eme ihe dị ka mkpụrụ ndụ onye na-ede akụkọ na bayoloji molecular iji chọpụta okwu mkpụrụ ndụ ihe nketa ma ọ bụ ọmụmụ ihe omume nkwalite.

Nyochaa Colony: A na-ejikarị X-Gal eme nyocha nyocha nke colony bacteria.Mpaghara nje na-egosipụta LacZ na-apụta na-acha anụnụ anụnụ mgbe ha toro na agar nwere X-Gal, na-eme ka njirimara dị mfe na nhọrọ nke mpaghara lacZ dị mma.

Nyocha Fusion Gene: A na-ejikwa X-Gal na nnwale njikọ mkpụrụ ndụ ihe nketa.Mgbe ejikọtara mkpụrụ ndụ ihe ezubere iche na mkpụrụ ndụ lacZ, ntụpọ X-Gal nwere ike ikpughe ụkpụrụ nke protein njikọ n'ime cell ma ọ bụ anụ ahụ.

Mpaghara Protein: Enwere ike iji ntụpọ X-Gal mee nyocha nhazi protein subcellular.Site na ijikọta protein nke mmasị na mkpụrụ ndụ lacZ, ọrụ β-galactosidase nwere ike igosi ebe protein dị n'ime cell.

X-Gal Analogues: Ụdị X-Gal gbanwetụrụ, dị ka Bluo-Gal ma ọ bụ Red-Gal, ka emepụtara iji nye ohere maka atụmatụ mmepe agba ọzọ.Ihe analogues ndị a na-eme ka ọdịiche dị n'etiti lacZ-positive na lacZ-negative cell ma ọ bụ anụ ahụ na-eji agba dị iche iche.

Nlereanya ngwaahịa

7240-90-6-1
7240-90-6-2

Nkwakọ ngwaahịa:

6892-68-8-3

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CAS Mba. 7240-90-6
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