4-NITROPHENYL-ALPHA-D-MANNOPYRANOSIDE CAS:10357-27-4

Enzyme substrates: 4NPM nwere ike iji dị ka mkpụrụ maka enzymes dị iche iche, gụnyere glycosidases na enzymes metụtara ya.Enzymes ndị a na-ekewa njikọ glycosidic n'etiti mannose na 4NPM, na-ebute mwepụta nke nitrophenyl moiety.Enwere ike tụọ oke hydrolysis nke mkpụrụ osisi n'ụdị spectrophotometrically site na nyochaa nnabata nke otu nitrophenyl a tọhapụrụ n'otu ogologo ogologo.Nke a na-enye ndị nchọpụta ohere ịchọpụta ọrụ enzyme na kinetics.

Nyocha maka carbohydrate metabolism: Site na iji 4NPM dị ka mkpụrụ, ndị nchọpụta nwere ike ịmụ ọrụ nke enzymes na-etinye aka na metabolism carbohydrate, dị ka alfa-mannosidases.Enzymes ndị a na-eme ka njikọ glycosidic hydrolyze n'ime ogige ndị nwere mannose, na enwere ike tụọ ọrụ ha site na nyochaa ntọhapụ nke nitrophenyl moiety site na 4NPM.

Ọmụmụ Glycosylation: 4NPM nwekwara ike iji nyocha nyocha iji nyochaa enzymes na-etinye aka na usoro glycosylation.Glycosylation bụ usoro ijikọ mkpụrụ ndụ shuga na protein ma ọ bụ ụmụ irighiri ihe ndị ọzọ, ọtụtụ enzymes na-etinye aka na usoro a.Site na iji 4NPM dị ka mkpụrụ nke nnabata, ndị ọrụ nyocha nwere ike tụọ nnyefe nke mmiri shuga gaa na 4NPM site na enzymes akọwapụtara nke metụtara mmeghachi omume glycosylation.

Nyochaa maka ndị na-egbochi enzyme ma ọ bụ ndị na-arụ ọrụ: 4NPM nwere ike iji mee ihe na nyocha nyocha dị elu iji chọpụta ogige ndị na-egbochi ma ọ bụ mee ka enzymes dị iche iche rụọ ọrụ.Site n'inyocha mmetụta nke ogige ule na hydrolysis ma ọ bụ mgbanwe nke 4NPM site na enzymes ebumnuche, ndị nchọpụta nwere ike ịchọpụta ndị nwere ike ịgwọ ọrịa ma ọ bụ nyocha kemịkalụ bara uru maka ịmụ ọrụ enzyme.