4-Nitrophenyl beta-D-galactpyranoside CAS: 200422-18-0

Mmetụta: ONPG bụ ihe eji eme ihe kpọmkwem iji chọpụta ọnụnọ na ọrụ nke enzyme β-galactosidase.Mgbe β-galactosidase enzyme dị ma na-arụ ọrụ, ọ na-ekewa ONPG n'ime ngwaahịa abụọ: o-nitrophenol na ihe nrụpụta galactose.Ntọhapụ nke o-nitrophenol na-ebute mgbanwe agba odo, nke enwere ike tụọ ya site na iji spectrophotometer.

Ngwa: ONPG nwere ọtụtụ ngwa na bayoloji ihe omimi na nyocha biochemistry:

Mkpebi nke ọrụ β-galactosidase: A na-ejikarị ONPG tụọ na tụọ ọrụ nke β-galactosidase enzyme.Ọnụego nhazi nke o-nitrophenol, nke dabara na ọrụ enzyme, nwere ike tụọ spectrophotometrically.

Okwu mkpụrụ ndụ ihe nketa na ụkpụrụ: ONPG na-ejikarị na nnwale metụtara mkpụrụ ndụ ihe nketa na ọmụmụ iwu.A na-ejikarị ya eme ihe na nyocha protein fusion, dị ka usoro njikọ lacZ a na-ejikarị eme ihe, iji mụọ okwu nke mkpụrụ ndụ ihe nketa n'okpuru nchịkwa nke ndị na-akwalite kpọmkwem.Ọrụ beta-galactosidase a tụrụ n'iji ONPG na-enye nghọta n'ogo nke okwu mkpụrụ ndụ ihe nketa.

Nleba anya maka ọrụ β-galactosidase: ONPG nwere ike iji dị ka usoro nyocha colorimetric na teknụzụ DNA recombinant iji chọpụta ọnụnọ na ọrụ nke mkpụrụ ndụ LacZ, nke na-edekọ β-galactosidase.Usoro nyocha a na-enyere aka ịchọpụta clones nwere mkpụrụ ndụ ihe nketa mmasị.

Ọmụmụ kinetics nke Enzyme: ONPG bara uru n'ịmụ kinetics nke β-galactosidase enzyme.Site n'ịleta ọnụego mmeghachi omume nke enzyme-substrate na mkpokọta mkpụrụ dị iche iche, ọ ga-ekwe omume ikpebi paramita kinetic dị ka Michaelis-Menten constants (Km) na ọnụego mmeghachi omume kachasị (Vmax).