HEPES-Na CAS: 75277-39-3 Onye nrụpụta ọnụahịa

Ihe na-echekwa ihe: HEPES sodium nnu ka a na-ejikarị dị ka onye na-echekwa ihe n'ime nnwale nke ndu yana biochemical.Ọ na-enyere aka ịnọgide na-enwe pH kwụsiri ike, karịsịa na nsonazụ physiological (pH 7.2-7.6).Ikike nchekwa ya na-eme ka ọ baa uru n'idebe ọnọdụ kwesịrị ekwesị maka mmeghachi omume enzymatic dị iche iche, omenala cell, na usoro ihe ọmụmụ ihe omimi.

Omenala cell: HEPES sodium nnu ka a na-ejikarị dị ka onye na-echekwa ihe na mgbasa ozi omenala cell.Ikike idowe pH kwụsiri ike dị mkpa maka uto kwesịrị ekwesị na ịdị ndụ nke mkpụrụ ndụ.A na-ahọrọ HEPES karịa ndị na-enyefe ihe ndị ọzọ ebe ọ bụ na ọ naghị egosi mgbanwe dị ukwuu na pH mgbe ekpughere ya na ikuku CO2.

Ọmụmụ ihe ọmụmụ: HEPES sodium nnu bara uru karịsịa na ọmụmụ enzymatic ebe a chọrọ ebe pH na-achịkwa mgbe niile.Ọ na-egbochi mgbanwe dị egwu na pH n'oge mmeghachi omume enzymatic, na-eme ka ọrụ enzyme kacha mma.

Electrophoresis: HEPES sodium nnu ka a na-ejikarị dị ka onye na-echekwa ihe na usoro electrophoretic dị iche iche dị ka polyacrylamide gel electrophoresis (PAGE) na agarose gel electrophoresis.Ọ na-enyere aka ịnọgide na-enwe pH nke ihe nchekwa, nke dị oké mkpa maka ikewapụ na nyochaa acid nucleic na protein.

Nnwale biochemical: A na-ejikarị nnu sodium HEPES eme ihe n'ọtụtụ nyocha biochemical, gụnyere nyocha enzyme, nyocha ọnụọgụ protein, na nyocha spectrophotometric.Ọ na-enyere aka idowe oke pH chọrọ maka nsonaazụ ziri ezi na enwere ike imepụtaghachi.

Nhazi ọgwụ: A na-ejikwa nnu sodium HEPES mee ihe n'ụdị ọgwụ dị ka onye na-echekwa ọgwụ iji kwado usoro ọgwụ na idowe oke pH chọrọ.