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Tapso Sodium CAS:105140-25-8 Ọnụahịa Onye Mmepụta
3-[N-Tris(hydroxymethyl)methylamino]-2-hydroxypropanesulfonic acid,Ọ bụ mihe na-egbochi ihe nfụkasị zwitterionic n'ụdị nnu sodium. A na-ejikarị ya eme ihe na nnwale biochemical na molekul bayọlọji maka ịnọgide na-enwe pH kwụsiri ike na ngwọta.It ọ na-agbaze nke ukwuu n'ime mmiri ma nwee pKa dị nso na pH physiological, nke na-eme ka ọ dabara adaba maka ọtụtụ ojiji dịka nhicha protein, nnwale enzyme, ọdịbendị sel, na electrophoresis. Nhazi kemịkalụ pụrụ iche ya na ndakọrịta ya na sistemụ bayoloji na-eme ka ọ bụrụ ihe dị mkpa na usoro nyocha dị iche iche.
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4-Nitrophenyl phosphate di(tris) nnu CAS:68189-42-4
Nnu 4-Nitrophenyl phosphate di(tris) bụ ihe mejupụtara kemịkalụ eji eme nnyocha na nchọpụta ihe ndị dị ndụ. A na-ejikarị ya eme ihe dị ka ihe ndabere maka nnwale enzyme alkaline phosphatase. Nnu a, mgbe alkaline phosphatase tinyere ya na hydrolyze, na-emepụta ngwaahịa agba odo nke enwere ike ịtụ nha spectrophotometric ngwa ngwa. A na-eji mmeghachi omume a eme ihe n'ọtụtụ ngwa dị iche iche, gụnyere enzyme-linked immunosorbent assays (ELISAs) na nchọpụta ọrụ phosphatase. Ike agba odo ahụ dabara kpọmkwem na ọrụ enzyme, na-enye ohere maka nha ziri ezi nke ọkwa alkaline phosphatase na ihe nlele bayọlọji. 4-Nitrophenyl phosphate di(tris) nnu kwụsiri ike, mmiri na-agbaze, ma nwee mmetụta dị ukwuu, na-eme ka ọ bụrụ nhọrọ a ma ama maka nnwale enzyme na nyocha bayọlọji na ahụike.
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Nitrotetrazolium acha anụnụ anụnụ chloride CAS:298-83-9
Nitrotetrazolium blue chloride, nke a makwaara dịka NBT ma ọ bụ NBT-Cl, bụ kemịkalụ a na-ejikarị eme ihe n'ule bayọlọji na nke biochemical. Ọ bụ ntụ kristal na-acha odo odo nke a na-agbaze n'ime mmiri.
A na-ejikarị NBT eme ihe dị ka ihe ngosi iji chọpụta ọnụnọ nke radicals superoxide ma ọ bụ enzymes ndị na-emepụta ha. Ọ na-agbanwe agba mgbe radicals superoxide belatara, na-agbanwe site na odo gaa na ihe na-akpalite formazan miri emi. A na-ejikarị mmeghachi omume a eme ihe na nnwale iji mụọ nrụgide oxidative, iku ume sel, na ọrụ enzyme.
A na-ejikarị NBT eme ihe n'ọtụtụ ụzọ dị iche iche, dịka mbelata nitroblue tetrazolium, iji hụpụta mmepụta superoxide na mkpụrụ ndụ na anụ ahụ. A na-ejikwa ya na immunohistochemistry na histochemistry iji chọpụta ma chọpụta enzymes na ihe ndị ọzọ dị na sel.
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NSP-AS CAS:211106-69-3 Ọnụahịa Onye Mmepụta
Nnu dị n'ime nke 3-[9-(((3-(carboxypropyl)[4-Methxylphenyl]sulfonyl)aMine)carboxyl]-10-acridiniuMyl)-1-propanesulfonate bụ molekul dị mgbagwoju anya nke nwere ihe dị n'ime acridinium nke ejikọtara na nnu dị n'ime nke propanesulfonate. O nwere otu carboxypropyl, otu 4-Methxylphenylsulfonyl, na otu amine. O yikarịrị ka a na-emepụta ihe a maka ojiji ụfọdụ na mpaghara dịka kemistri, ọgwụ, ma ọ bụ nyocha.
Ọzọkwa, NAC egosila nkwa n'ịkwado ahụike imeju site n'inyere aka n'iwepụ ihe ndị na-egbu egbu, dị ka acetaminophen, ọgwụ mgbu a na-ahụkarị. O nwekwara ike inwe mmetụta nchebe megide mmebi imeju nke mmanya na-aba n'anya kpatara.
Tinyere ihe ndị na-egbochi ọrịa na iku ume, a chọpụtala uru NAC nwere ike ịba n'ahụike uche. Ụfọdụ nnyocha na-egosi na ọ nwere ike inwe mmetụta dị mma na nsogbu ọnọdụ uche, dịka ịda mbà n'obi na nsogbu nrụgide uche (OCD).
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L-Leucine p-nitroanilide hydrochloride CAS: 16010-98-3
L-Leucine p-nitroanilide hydrochloride bụ kemịkalụ nke so na otu ihe ndị sitere na p-nitroanilide. Ọ bụ ntụ kristal ọcha site na ọcha ruo na ọcha nke a na-ejikarị eme ihe na nyocha biochemical na nnwale enzyme.
A na-ejikarị L-Leucine p-nitroanilide hydrochloride eme ihe dị ka ihe e ji amịpụta ihe iji tụọ ọrụ nke enzymes dị ka proteases na peptidases. Mgbe enzymes ndị a kewara L-Leucine p-nitroanilide hydrochloride, ọ na-ewepụta p-nitroaniline, nke enwere ike ịchọpụta site na spectrophotometric. Ọnụego mgbanwe agba ahụ kwekọrọ kpọmkwem na ọrụ enzymatic, na-enye ohere maka ịtụle ọrụ enzyme na ihe nlele bayọlọji.
A na-agbaze ihe a n'ime mmiri ma nwee ike ịgbaze ya ngwa ngwa na mmiri nchekwa kwesịrị ekwesị maka iji ya na nnwale enzymatic. Ọ dị mkpa ịchekwa L-Leucine p-nitroanilide hydrochloride n'ebe dị jụụ ma kpọọ nkụ iji mee ka ọ kwụsie ike.
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Guanidine Thiocyanate Sulfate CAS:593-84-0
Guanidine thiocyanate sulfate bụ kemịkalụ sitere na ion guanidine, thiocyanate, na sulfate. A na-ejikarị ya eme ihe na bayọlọjik molekul na nyocha bayọlọjik dị ka ihe na-akpata nsogbu.
A na-ejikarị Guanidine thiocyanate sulfate eme ihe na usoro DNA na RNA iji mebie akpụkpọ ahụ sel na protein denature, na-enye ohere maka mwepụta nke nucleic acid nke ọma. Ọ na-enyere aka na ịgbaze DNA na RNA, na-eme ka ha dị ọcha ma na-eme ka ha dị ọcha site na ọtụtụ ihe atụ bayọlọji dị iche iche.
N'ihi ihe ndị dị na ya nke na-eme ka ọ dị ka chaotropic, guanidine thiocyanate sulfate na-enyere aka imebi njikọ hydrogen na mmekọrịta ndị ọzọ na-abụghị covalent, na-eme ka nkewa nke nucleic acid na protein, lipids, na ihe ndị ọzọ dị na sel dị mfe. Ọ na-enyekwa aka igbochi ọrụ RNase, na-ahụ na ọ dị ọcha ma na-eme ka a mata na ọ dị ọcha nke nucleic acid ndị a na-ewepụta..
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BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine nnu CAS:6578-06-9
BCIP-Toluidine, nke a makwaara dị ka nnu 5-Bromo-4-chloro-3-indolylphosphate-p-toluidine, bụ ihe mejupụtara kemịkalụ a na-ejikarị eme ihe na nchọpụta bayọlọjik na biochemistry. A na-ejikarị ya eme ihe dị ka ihe na-eme ka a mata ihe ndị ...
Mgbe alkaline phosphatase kewara BCIP-Toluidine, ọ na-enweta mgbanwe agba, na-emepụta ihe na-akpalite acha anụnụ anụnụ-acha odo odo. Mmeghachi omume a na-enye ohere maka ịhụ anya na nha nke ọrụ alkaline phosphatase na ihe nlele. Enwere ike ịtụ ike nke agba ahụ site na spectrophotometric ma ọ dabara kpọmkwem na ọrụ enzyme.
Ojiji BCIP-Toluidine dị ka ihe e ji eme ihe bụ ihe a na-ahụkarị n'ọrụ ndị metụtara DNA ma ọ bụ RNA labeling, dịka in situ hybridization (ISH) ma ọ bụ Southern na Northern blotting. Ọ na-enye ụzọ a pụrụ ịtụkwasị obi ma dị nro maka ịchọpụta ọnụnọ nke nucleic acid ndị a na-elekwasị anya na samples.
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3-Aminophthalhydrazide CAS:521-31-3
3-Aminophthalhydrazide bụ ihe mejupụtara kemịkalụ nwere usoro molekul C8H8N2O. A na-akpọ ya ihe sitere na aminohydrazide nke phthalic acid. Ihe mejupụtara a nwere azụ azụ phthalic acid nke nwere atọm hydrogen abụọ nke amino otu na otu hydrazine dochie.
3-Aminophthalhydrazide nwere ọtụtụ ojiji n'ọhịa nke kemistri na ọgwụ organic. Enwere ike iji ya dị ka ihe mmalite na mmepụta nke ọtụtụ ihe ọgwụ na agba. Na mgbakwunye, a na-ejikarị ya eme ihe na nkwadebe nke ntọala Schiff, nke bụ ihe dị mkpa na mmepụta organic.
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4-Aminoantipyrine CAS:83-07-8
4-Aminoantipyrine, nke a makwaara dị ka aminoantipyrine ma ọ bụ aminopyrine, bụ ihe mejupụtara ihe ndị e ji emepụta ihe. A na-ejikarị ya eme ihe dị ka ihe na-eme ka ihe dị n'ime kemịkalụ nyocha na ụlọ ọrụ ọgwụ. Nhazi kemịkalụ ya nwere mgbanaka aromatic nwere otu amino (NH2) na otu pyrazolone, nke na-eme ka ọ bụrụ ihe dị iche iche maka ojiji dị iche iche.
N'ihe gbasara kemistri nyocha, a na-eji 4-Aminoantipyrine eme ihe nke ukwuu dị ka ihe na-eme ka ihe dị iche iche dị ka chromogenic reagent na nyocha agba. Ọ na-emeghachi omume na ụfọdụ ihe, dị ka phenols na peroxides, iji mepụta ihe mgbagwoju anya agba nke enwere ike ịtụ site na spectrophotometric. Njirimara a na-eme ka ọ baa uru na nnwale dị iche iche, gụnyere mkpebi nke enzymes dị ka peroxidase, glucose, na amylase.
N'ụlọ ọrụ ọgwụ, ejirila 4-Aminoantipyrine mee ihe n'akụkọ ihe mere eme dị ka ọgwụ mgbu na ọgwụ mgbu. .
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TAPS-NA CAS:91000-53-2 Ọnụahịa Onye Mmepụta
N-[Tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid sodium salt, nke a makwaara dị ka TAPS-Na, bụ kemịkalụ a na-ejikarị eme ihe dị ka ihe na-egbochi ihe n'ọtụtụ ihe ndị dị ndụ na ihe ndị dị ndụ. Ọ bụ ihe na-eme ka zwitterionic dị na ya, nke pụtara na o nwere ma chajị dị mma ma nke na-adịghị mma, nke na-enyere aka ịnọgide na-enwe pH kwụsiri ike na ngwọta.
TAPS-Na nwere oke pH dị ihe dịka 7.7 ruo 9.1, nke mere ka ọ dabara adaba maka ojiji chọrọ gburugburu pH alkaline dị ntakịrị. A na-ejikarị ya eme ihe na nyocha protein na enzyme, yana na usoro bayoloji molekul dịka gel electrophoresis na usoro DNA.
Dịka nnu sodium, TAPS-Na na-agbaze nke ukwuu n'ime mmiri, nke na-eme ka ọ dị mfe ịkwadebe ihe ngwọta ndị a na-echekwa. A maara ya maka obere nsí na obere nnyonye anya na molekul ndị dị ndụ, nke na-eme ka ọ bụrụ nhọrọ a na-ahọrọ maka ọtụtụ nnwale.
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5,5-Dithiobis(2-nitrobenzoic acid) CAS:69-78-3
5,5-Dithiobis (2-nitrobenzoic acid), nke a makwaara dị ka DTNB ma ọ bụ ihe na-eme ka Ellman dị nro, bụ ihe mejupụtara kemịkalụ a na-ejikarị eme nchọpụta kemịkalụ na ọgwụ. Ọ bụ ntụ ntụ kristal odo odo nke a na-agbaze nke ukwuu na mmiri na ihe mgbaze organic.
A na-ejikarị DTNB atụ nha nke otu sulfhydryl (-SH) na protein na peptides. Ngwakọta ahụ na-emeghachi omume na thiols, na-eme ka e nwee ngwakọta disulfide ma wepụta 2-nitro-5-thiobenzoate, nke nwere agba odo nke enwere ike ịtụ spectrophotometric. Site n'ịtụle ike nke agba odo ahụ, ọ ga-ekwe omume ịtụle mkpokọta thiols na ihe nlele. A na-ejikarị usoro a amụ mmekọrịta protein na protein, mpịachi protein, na mmeghachi omume enzymatic nke gụnyere otu thiol.
Na mgbakwunye na nhazi thiol, ejirila DTNB mee ihe n'ọrụ ndị ọzọ, dịka inyocha ọnọdụ redox nke mkpụrụ ndụ, ịmụ nkwụsi ike nke ihe mejupụtara ígwè na sọlfọ, na ịtụ ọrụ nke enzymes dị iche iche.
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Phenolphthalein monophosphate BIS(Cyclohexylammonium) Nnu CAS:14815-59-9
Nnu Phenolphthalein monophosphate dicyclohexylammonium bụ ihe mejupụtara kemịkalụ nke sitere na phenolphthalein, ihe ngosi pH a na-ejikarị eme ihe na ntọala ụlọ nyocha. A na-ejikarị ụdị nnu dicyclohexylammonium nke phenolphthalein monophosphate dị ka ihe nyocha na nyocha bayoloji na molekul. A na-ejikarị ya eme ihe na nnwale ma ọ bụ nnwale iji chọpụta ma tụọ ọnụnọ nke ụfọdụ enzymes, gụnyere ndị metụtara ATP (adenosine triphosphate) hydrolysis ma ọ bụ phosphatase. Njirimara pụrụ iche ya na-eme ka ọ bụrụ ngwa ọrụ bara uru na nyocha sayensị metụtara kinetics enzyme na usoro sel..
