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Vancomycin hydrochloride CAS:1404-93-9
Vancomycin hydrochloride bụ ọgwụ nje e ji agwọ ọrịa ndị siri ike nke ụfọdụ nje bacteria na-akpata. Ọ bụ nke otu glycopeptide nke ọgwụ ma na-arụ ọrụ site n'igbochi mmepụta nke peptidoglycan, ihe dị mkpa nke mgbidi mkpụrụ ndụ nje bacteria. A na-ejikarị ya agwọ ọrịa ndị dị ka MRSA, afọ ọsịsa Clostridium difficile, na endocarditis nke nje bacteria..
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HEPPS CAS:16052-06-5 Ọnụahịa Onye Mmepụta
HEPPS, nke na-anọchi anya N-(2-Hydroxyethyl)piperazine-N'-2-ethanesulfonic acid, bụ ihe na-eme ka ihe dị n'ime zwitterionic na-arụ ọrụ nke a na-ejikarị eme nnyocha gbasara ihe ndị dị ndụ na ihe ndị dị ndụ. A maara ya maka ikike nchekwa ya dị mma na nkwụsi ike ya n'elu oke pH sara mbara, ọkachasị na oke pH 6.8-8.2. A na-ejikarị HEPPS eme ihe na mgbasa ozi omenala mkpụrụ ndụ, nhicha protein, mmeghachi omume enzymatic, na ngwa ndị ọzọ chọrọ gburugburu pH a na-achịkwa nke ọma. A na-agbaze ya na mmiri ma a na-ejikarị ya eme ihe na oke dị site na 10 mM ruo 100 mM. A na-ewere HEPPS dị ka ihe na-adịghị egbu egbu nye mkpụrụ ndụ ma nwee obere nnyonye anya ndabere, na-eme ka ọ bụrụ nhọrọ a ma ama maka ọtụtụ usoro nnwale.
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2,3,5-Triphenyl-2H-tetrazolium chloride CAS:298-96-4
2,3,5-Triphenyl-2H-tetrazolium chloride, nke a makwaara dị ka TTC, bụ ihe a na-ejikarị eme ihe dị ka ihe ngosi redox n'ihe gbasara ihe ndị dị ndụ na kemịkalụ. Ọ na-apụta dị ka ntụ kristal na-acha odo odo na ọcha.
A na-ejikarị TTC eme ihe n'ọtụtụ nnwale na nnwale dị iche iche iji chọpụta ndụ mkpụrụ ndụ na ọrụ metabolic. Mgbe etinyere ya na mkpụrụ ndụ dị ndụ, a na-ebelata ya site na enzymatic iji mepụta ngwaahịa formazan uhie. Mbelata a dabere na enzymes dehydrogenase na-arụ ọrụ dị na mkpụrụ ndụ ndị dị ndụ. N'ihi ya, ọnụọgụ formazan uhie emepụtara kwekọrọ na ọnụọgụ sel ndị na-arụ ọrụ metabolic.
Ike TTC nwere inyocha ndụ mkpụrụ ndụ na-eme ka ọ baa uru n'ebe nnyocha dịka nnwale nsí cytotoxicity, nnwale mmụba mkpụrụ ndụ, na nyocha nke irè ọgwụ. Na mgbakwunye, a na-eji ya eme ihe na microbiology maka inyocha uto nje bacteria, yana n'ọmụmụ ihe gbasara ọdịbendị anụ ahụ osisi na nyocha ndụ anụ ahụ.
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Gly-Pro p-nitroanilide p-toluenesulfonate nnu CAS:65096-46-0
Nnu Gly-Pro p-nitroanilide p-toluenesulfonate bụ ihe mejupụtara kemịkalụ eji eme nnwale enzymatic iji tụọ ọrụ nke peptidases proline. Ọ mejupụtara dipeptide Gly-Pro nke ejikọtara na otu p-nitroanilide, nke enwere ike iwepụta site na ọrụ nke peptidases proline-specific.
N'ime nnwale enzymatic, a na-agbakwunye ihe mejupụtara a na ihe nlele nwere enzyme nke mmasị. Enzyme ahụ na-ekewa njikọ dị n'etiti glycine (Gly) na proline (Pro), na-ewepụta otu p-nitroanilide. Enwere ike ịtụ p-nitroanilide ewepụtara site na iji spectrophotometric, na-enye ohere ịchọpụta ọrụ enzyme ahụ.
Ụdị nnu p-toluenesulfonate nke Gly-Pro p-nitroanilide na-enye nkwụsi ike na mmiri mmiri, na-eme ka ojiji ya dị mfe na nnwale enzymatic..
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5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide CAS:4264-82-8
5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide bụ ihe e ji eme nnyocha dị iche iche gbasara ihe ndị dị ndụ, ọkachasị maka ịchọpụta na ịhụ ihe na-eme na ọrụ enzyme. Ọ bụ ihe e ji emepụta ihe nke enzymes pụrụ iche nwere ike ime ka ọ dị mmiri mmiri, nke na-eme ka e wepụta ngwaahịa nwere agba ma ọ bụ fluorescent.
A na-ejikarị ihe mejupụtara a eme ihe n'ule iji chọpụta ọnụnọ na ọrụ nke enzymes dịka beta-galactosidase na beta-glucuronidase. Enzymes ndị a na-ekewapụ otu acetyl na glucosaminide site na substrate, na-eduga n'ịmepụta chromophore na-acha anụnụ anụnụ ma ọ bụ akwụkwọ ndụ akwụkwọ ndụ.
Nhazi pụrụ iche nke 5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide na-enye ohere maka nchọpụta na nhazi nke ọrụ enzyme dị mfe. Ojiji ya na usoro nnwale dị iche iche, gụnyere histochemistry, immunohistochemistry, na nnwale dabere na mkpụrụ ndụ, enyela aka na nghọta ka mma nke ọrụ enzyme.
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AMPPD CAS:122341-56-4 Ọnụahịa Onye Mmepụta
AMPPD, mkpụmkpụ maka 3-(2′-spiroadamantane)-4-methoxy-4-(3″-phosphoryloxy)phenyl-1,2-dioxetane, bụ ihe e ji emepụta chemiluminescent nke a na-ejikarị eme ihe n'ihe gbasara bayoloji na immunoassay. A na-ejikarị ya eme ihe n'ọtụtụ ebe na nchọpụta bayoloji molekul, nchọpụta ọrịa, na sayensị ndụ.
E mere AMPPD kpọmkwem ka e jiri ya na njikọta nke alkaline phosphatase enzyme conjugates. Mgbe e tinyere ya na alkaline phosphatase, AMPPD na-enweta mmeghachi omume enzyme dị ngwa ma dị irè, nke na-eduga na mwepụta nke ìhè.
A na-eji sistemụ onyonyo ma ọ bụ ihe nrịbama dị nro achọpụta ìhè sitere na AMPPD, nke na-enye ohere maka nha na ịhụ ihe ndị a na-ahụ anya nke molekul ma ọ bụ ihe nyocha. Usoro nchọpụta dabere na chemiluminescence a na-enye nnukwu mmetụta, obere mkpọtụ ndabere, na oke ike dị iche iche.
N'ihi nkwụsi ike ya na mgbaàmà ya na-adịte aka, AMPPD dabara nke ọma maka ojiji dịka Western blotting, ELISA (enzyme-linked immunosorbent assay), DNA hybridization, na in situ hybridization. Ọ na-enye ndị nchọpụta ohere ịchọpụta ma nyochaa protein, nucleic acid, antibodies, na biomolecules ndị ọzọ.
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2,6-Dichloroindophenol sodium nnu CAS:620-45-1
3,5-Dichlorophenyldiazonium tetrafluoroborate bụ kemịkalụ a na-ejikarị eme ihe na njikọ organic. Ọ bụ nnu diazonium, nke pụtara na o nwere otu diazonium (N2+) nke ejikọtara na mgbanaka phenyl nke ejiri atọm chlorine dochie ya na ọnọdụ nke atọ na nke ise.
A na-ejikarị ihe mejupụtara a eme ihe n'ihe gbasara mmeghachi omume ndị metụtara ijikọ ya na ihe ndị ọzọ na-esi ísì ụtọ. Ọ nwere ike ịnweta mmeghachi omume dịka mgbanwe aromatic electrophilic, ebe ọ nwere ike ibufe otu diazonium ya gaa na ihe ọzọ na-esi ísì ụtọ nucleophilic iji mepụta njikọ ọhụrụ. Nke a bara uru karịsịa maka iwebata otu ọrụ na mgbanaka aromatic..
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3,3′-Diaminobenzidine CAS:91-95-2
3,3′-Diaminobenzidine (DAB) bụ kemịkalụ a na-ejikarị eme ihe na usoro immunohistochemistry (IHC) na immunocytochemistry (ICC). Ọ bụ ihe na-emepụta chromogenic substrate nke na-emepụta ngwaahịa mmeghachi omume aja aja mgbe ekpughere ya na enzymes peroxidase, nke na-egosi ọnụnọ nke antigen a na-elekwasị anya.
A na-ejikarị DAB eme ihe iji hụta protein ma ọ bụ biomolecules ndị ọzọ nwere mmasị n'ime ihe atụ bayọlọji, dịka akụkụ anụ ahụ ma ọ bụ mkpụrụ ndụ. Ọ nwere ike inye akara mpaghara na nke a na-ahụ anya maka antigen a na-elekwasị anya, na-enye ndị nchọpụta ohere ịchọpụta ma nyochaa nkesa ya na ịba ụba ya.
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(±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid CAS:53188-07-1
Trolox bụ ihe mejupụtara sịntetik nke so na otu antioxidants a maara dị ka vitamin E analogs. Ọ bụ ihe antioxidant siri ike nke nwere ike ịchụpụ radicals efu ma chebe mkpụrụ ndụ na anụ ahụ site na mmebi oxidative. A mụọla Trolox nke ọma maka uru ahụike ya, gụnyere ikike ya ibelata mbufụt, igbochi mmebi sel, na ịkwado ahụike obi. A na-ejikarị ya eme ihe dị ka ihe ntụnyere na nyocha antioxidant yana dị ka ihe mgbakwunye na ngwaahịa nri na ihe ịchọ mma dị iche iche.
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1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride CAS:25952-53-8
1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, nke a makwaara dịka EDC hydrochloride ma ọ bụ EDAC, bụ kemịkalụ a na-ejikarị eme ihe na biochemistry na bayoloji molekul dị ka ihe na-ejikọ aka maka ime ka otu carboxyl rụọ ọrụ. Ọ bụ ntụ kristal ọcha nke na-agbaze nke ukwuu na mmiri.
A na-ejikarị EDC hydrochloride eme ihe n'ịmepụta peptides na protein site n'inyere aka ịmepụta njikọ amide n'etiti carboxyl na otu amino. Ọ na-arụ ọrụ site n'ịkwalite otu carboxyl, nke na-emeghachi omume na otu amino n'ihu ihe na-ejikọ dịka N-hydroxysuccinimide (NHS). Mmeghachi omume a na-eme ka njikọ amide kwụsie ike.
A na-ejikwa EDC hydrochloride eme ihe na nchọpụta DNA na RNA maka ịgbanwe ma jikọta nucleic acid, yana ime ka enzymes na biomolecules ndị ọzọ ghara ịdị n'elu. Ọ bụ ihe na-agbanwe agbanwe nke nwere ike ime ka arụmọrụ na nkọwapụta nke mmeghachi omume ndị a ka mma.
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HEPES CAS:7365-45-9 Ọnụahịa Onye Mmepụta
HEPES (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid) bụ ihe nchekwa bayọlọji nke a na-ejikarị eme nnyocha bayọlọji na kemịkalụ. A maara ya maka ikike ya ịnọgide na-enwe pH kwụsiri ike na mmiri mmiri, na-eme ka ọ dabara adaba maka ojiji dị iche iche gụnyere usoro sel na enzymatic. HEPES bụ ihe mejupụtara zwitterionic, nke pụtara na ọ nwere ebubo dị mma na nke na-adịghị mma na otu ọrụ dị iche iche, nke na-enye ya ohere iguzogide mgbanwe na pH nke mgbakwunye nke asịd ma ọ bụ ntọala kpatara. A na-ejikarị ya eme ihe na omenala sel, nnwale enzyme, ọmụmụ protein, nnwale electrophoresis, na usoro ọgwụ.
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p-Nitrophenyl -aL-Fucopyranoside CAS:10231-84-2
p-Nitrophenyl-α-L-Fucopyranoside bụ ihe mejupụtara kemịkalụ nke sitere na klas nke nitrophenyl glycosides. A na-ejikarị ya eme ihe na nyocha biochemical dị ka ihe ndabere maka enzyme α-L-fucosidase, nke na-eme ka hydrolysis ya ka mma. Ihe mejupụtara a enweghị agba ma na-agbaze na ihe mgbaze organic, na-eme ka ọ dị mfe maka nnwale enzymatic.
