Phenylgalactoside CAS: 2818-58-8

Mmetụta na ọrụ enzyme: Phenylgalactoside na-ejikarị tụọ ọrụ nke enzyme β-galactosidase.Mgbe phenylgalactoside na-hydrolyzed site β-galactosidase, ọ na-ahapụ p-nitrophenol.Enwere ike ịlele mkpokọta p-nitrophenol n'ụzọ dị iche iche, na-enye nghọta na ọrụ β-galactosidase.A na-etinye mmetụta a na ngwa ndị dị ka nyocha enzyme na usoro nyocha.

Nyocha okwu mkpụrụ ndụ ihe nketa: A na-ejikarị Phenylgalactoside mee ihe dị ka mkpụrụ n'ime nyocha ihe ọmụmụ ihe ọmụmụ iji mụọ okwu mkpụrụ ndụ ihe nketa.A na-ejikọta mkpụrụ ndụ lacZ, nke na-edekọ β-galactosidase, yana usoro nhazi nke mkpụrụ ndụ ihe nketa ndị ọzọ nwere mmasị.Okwu nke mkpụrụ ndụ lacZ na hydrolysis nke phenylgalactoside site na β-galactosidase nwere ike igosi usoro ngosipụta na ọkwa nke mkpụrụ ndụ ebumnuche a na-amụ.

Sistemụ nyocha: Phenylgalactoside bụ akụkụ dị mkpa nke sistemu nyocha nke na-eji ọrụ β-galactosidase.Otu ihe atụ a ma ama bụ usoro nyocha nke na-acha anụnụ anụnụ na-acha ọcha, nke a na-eji achọpụta sel recombinant ma ọ bụ gbanwee n'ime nyocha ihe ọmụmụ ihe omimi.Obodo ndị nwegoro nke ọma na DNA recombinant ma ọ bụ nwetaghachi mkpụrụ ndụ ihe nketa ga-egosipụta β-galactosidase, na-eduga na hydrolysis nke phenylgalactoside na nhazi nke agba anụnụ anụnụ.

Protein dị ọcha: N'ọnọdụ ụfọdụ, enwere ike iji phenylgalactoside dị ka njikọ maka chromatography mmekọrịta iji sachaa protein ndị na-ejikọta kpọmkwem ma ọ bụ na-arụ ọrụ site na β-galactosidase.Protein nke mmasị nwere ike ịnwe mkpado mmekọ ma ọ bụ mkpado ngwakọta nwere ngalaba β-galactosidase-jide.Site n'ịfefe ngwakọta protein site na kọlụm nwere phenylgalactoside na-enweghị ike imegharị ya, enwere ike ijikwa protein a chọrọ ma mechaa gbachapụ ya.